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ZFIN ID: ZDB-FIG-140325-17
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6

Notch3 is sufficient to promote brain pericyte proliferation. (A,B) N3ICD-EGFP overexpression increased the number of pdgfrb+ brain pericytes at 72 hpf. (A) Non-transgenic control larvae. (B) Tg(hsp70I:N3ICD-EGFP) larva heat-shocked (HS) at 48 hpf. (C,D) N3ICD overexpression rescued the brain pericyte deficit phenotype in notch3fh332 mutant larvae at 72 hpf. (C) notch3fh332 homozygous larvae heat-shocked at 48 hpf. (D) notch3fh332 homozygous larvae carrying the Tg(hsp70I:N3ICD-EGFP) transgene and heat shocked at 48 hpf. Arrowheads, pdgfrb+ brain pericytes. (E) Quantification of pdgfrb+ brain pericytes in control and N3ICD fish at 72 hpf (n=30 each). (F) Quantification of brain pericytes and percentage of EdU+ pericytes in control and N3ICD fish heat-shocked at 72 hpf and EdU pulsed at 78 hpf (n=25 each). (G) Quantification of brain pericytes at 72 hpf in control larvae, N3ICD larvae heat-shocked at 48 hpf, control larvae treated with 25 µM AG1295, and N3ICD larvae heat-shocked at 48 hpf immediately followed by AG1295 treatment (ne20 each). (H) Quantification of brain pericytes at 72 hpf in notch3fh332 larvae heat shocked at 48 hpf (control group) and notch3fh332;Tg(hsp70I: N3ICD-EGFP) larvae heat shocked at 48 hpf. Error bars represent s.d. *P<0.001 by Student’s two-tailed t-test. Scale bar: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP co17Tg; y1Tg standard conditions Protruding-mouth cranial blood vessel IFL
co17Tg; y1Tg heat shock Protruding-mouth cranial blood vessel IFL
notch3fh332/fh332; co17Tg; y1Tg heat shock Protruding-mouth cranial blood vessel IFL
notch3fh332/fh332; y1Tg heat shock Protruding-mouth cranial blood vessel IFL
y1Tg standard conditions Protruding-mouth cranial blood vessel IFL
pdgfrb co17Tg; y1Tg heat shock Protruding-mouth cranial blood vessel pericyte ISH
notch3fh332/fh332; co17Tg; y1Tg heat shock Protruding-mouth cranial blood vessel pericyte ISH
notch3fh332/fh332; y1Tg heat shock Protruding-mouth cranial blood vessel pericyte ISH
y1Tg standard conditions Protruding-mouth cranial blood vessel pericyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
co17Tg; y1Tg heat shock Long-pec cranial blood vessel pericyte increased amount, abnormal
Protruding-mouth cranial blood vessel pericyte increased amount, abnormal
notch3fh332/fh332; co17Tg; y1Tg heat shock Protruding-mouth cranial blood vessel pericyte normal amount, normal
notch3fh332/fh332; y1Tg heat shock Protruding-mouth cranial blood vessel pericyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development