ZFIN ID: ZDB-FIG-140325-17
Notch3 is sufficient to promote brain pericyte proliferation. (A,B) N3ICD-EGFP overexpression increased the number of pdgfrb+ brain pericytes at 72 hpf. (A) Non-transgenic control larvae. (B) Tg(hsp70I:N3ICD-EGFP) larva heat-shocked (HS) at 48 hpf. (C,D) N3ICD overexpression rescued the brain pericyte deficit phenotype in notch3fh332 mutant larvae at 72 hpf. (C) notch3fh332 homozygous larvae heat-shocked at 48 hpf. (D) notch3fh332 homozygous larvae carrying the Tg(hsp70I:N3ICD-EGFP) transgene and heat shocked at 48 hpf. Arrowheads, pdgfrb+ brain pericytes. (E) Quantification of pdgfrb+ brain pericytes in control and N3ICD fish at 72 hpf (n=30 each). (F) Quantification of brain pericytes and percentage of EdU+ pericytes in control and N3ICD fish heat-shocked at 72 hpf and EdU pulsed at 78 hpf (n=25 each). (G) Quantification of brain pericytes at 72 hpf in control larvae, N3ICD larvae heat-shocked at 48 hpf, control larvae treated with 25 µM AG1295, and N3ICD larvae heat-shocked at 48 hpf immediately followed by AG1295 treatment (ne20 each). (H) Quantification of brain pericytes at 72 hpf in notch3fh332 larvae heat shocked at 48 hpf (control group) and notch3fh332;Tg(hsp70I: N3ICD-EGFP) larvae heat shocked at 48 hpf. Error bars represent s.d. *P<0.001 by Student’s two-tailed t-test. Scale bar: 200 μm.
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