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ZFIN ID: ZDB-FIG-140325-15
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 4

notch3fh332 mutants have a deficit of pericytes. (A-F) pdgfrb+ brain pericytes in wild-type (WT) and mutant fish. Arrowheads, pdgfrb+ pericytes in the mutant larvae; open arrowheads, reduced vessel branching in forebrain and hindbrain. All images show confocal projections of whole brains from lateral view with dorsal up and anterior to the left. (G) Quantification of pdgfrb+ brain pericytes at different time points during embryonic and larval development in wild-type and mutant fish (ne25 each). *P<0.001 by Student’s two-tailed t-test. Scale bar: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP notch3fh332/fh332; y1Tg standard conditions Long-pec cranial blood vessel IFL
Protruding-mouth cranial blood vessel IFL
Day 4 cranial blood vessel IFL
y1Tg standard conditions Long-pec cranial blood vessel IFL
Protruding-mouth cranial blood vessel IFL
Day 4 cranial blood vessel IFL
pdgfrb notch3fh332/fh332; y1Tg standard conditions Long-pec cranial blood vessel pericyte ISH
Protruding-mouth cranial blood vessel pericyte ISH
Day 4 cranial blood vessel pericyte ISH
y1Tg standard conditions Long-pec cranial blood vessel pericyte ISH
Protruding-mouth cranial blood vessel pericyte ISH
Day 4 cranial blood vessel pericyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
notch3fh332/fh332; y1Tg standard conditions Long-pec cranial blood vessel pericyte decreased amount, abnormal
Protruding-mouth cranial blood vessel pericyte decreased amount, abnormal
Day 4 cranial blood vessel pericyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development