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ZFIN ID: ZDB-FIG-140325-12
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 1

pdgfrb expression marks brain pericytes in zebrafish embryos and larvae. (A,B) pdgfrb expression detected by ISH in trunk vasculature. Black arrowheads, DLAV; white arrowheads, DA; arrow, ISV. (C) pdgfrb expression in head vessels. Black arrowhead, primary head sinus; white arrows, pdgfrb+ cells associated with central arteries. Inset shows a high magnification view of pdgfrb+ cells in the brain. (D) Fluorescence ISH showing pdgfrb expression in trunk vessels (arrowheads). (E,F) Confocal sections showing pdgfrb expression in the hindbrain vasculature at 56 and 72 hpf, respectively. Arrowheads, Y-shape cell body of a pdgfrb+ pericyte; arrows, sprouting endothelial tip cells; open arrowhead, spindle-like cell body of a pdgfrb+ pericyte. Insets show a high magnification view of the pdgfrb+ pericytes. (G,H) Diagrams depicting hindbrain vasculatures at 56 hpf (G) and 72 hpf (H). (I-L) Confocal projections showing pdgfrb+ pericytes in the entire brain at 48, 56, 72 and 80 hpf. (M) Quantification of pdgfrb+ brain pericytes at different time points during embryonic and larval development (48 hpf and 56 hpf, n=15 each; other time points, n=30 each). (N) pH3+ pdgfrb+ brain pericyte at 78 hpf. (O) EdU+ pdgfrb+ brain pericyte at 78 hpf. (P) Percentage of EdU+ brain pericytes labeled by a 1-hour pulse at 54 hpf and 78 hpf (n=15 each). Error bars represent s.d. BA, basal artery; CtA, central artery; DA, dorsal aorta; ISV, intersegmental vessels; PHBC, primary hindbrain channels. Scale bars: 0.5 mm (A,B); 250 ┬Ám (C); 200 μm (D-F,I-L); 40 μm (N,O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Long-pec central artery IFL
Long-pec primordial hindbrain channel IFL
Protruding-mouth central artery IFL
Protruding-mouth dorsal aorta IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth primordial hindbrain channel IFL
pdgfrb WT standard conditions Long-pec dorsal aorta ISH
Long-pec dorsal longitudinal anastomotic vessel ISH
Protruding-mouth central artery ISH
Protruding-mouth dorsal aorta ISH
Protruding-mouth dorsal longitudinal anastomotic vessel ISH
Protruding-mouth intersegmental vessel ISH
Protruding-mouth primary head sinus ISH
y1Tg standard conditions Long-pec central artery ISH
Long-pec central artery pericyte ISH
Long-pec primordial hindbrain channel ISH
Long-pec primordial hindbrain channel pericyte ISH
Protruding-mouth central artery ISH
Protruding-mouth central artery pericyte ISH
Protruding-mouth dorsal aorta ISH
Protruding-mouth intersegmental vessel ISH
Protruding-mouth primordial hindbrain channel ISH
Protruding-mouth primordial hindbrain channel pericyte ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development