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ZFIN ID: ZDB-FIG-140325-18
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. S1

Expression patterns of tagln2 and pdgfrb in zebrafish embryos and larvae. (A-C) ISH for tagln2. (A,B) tagln2 expression in trunk vessels at 48 hpf and 72 hpf, respectively. Arrowhead, tagln2+ putative mural cell in ISV. (C,D) tagln2 expression in brain vasculature at 48 hpf and 72 hpf. Arrow, tagln2+ putative brain mural cell. Inset, high magnification view of tagln2+ cells in the brain. (E-L) desmina, desminb, rgs5a, and cspg4 expression in trunk and brain vasculature at 72 hpf. (E,G,I,K) trunk expression. (F,H,J,L) brain expression. (M) Fluorescent ISH showing pdgfrb expression in retinal pericytes at 72 hpf. Arrowheads, pdgfrb+ retinal pericytes. (N) Quantification of pdgfrb+ pericytes associated with different hindbrain vessels at various time points. (O,P) foxd3 MO knockdown caused reduction in pdgfrb+ brain pericyte number. (O) Control MO-injected 72 hpf larva. (P) foxd3 MO-injected 72 hpf larva. Arrowheads, pdgfrb+ brain pericytes. HA, hyaloid artery; HV, hyaloid vessels. Scale bars: (A-L) 0.5 mm, (M-P) 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cspg4 WT standard conditions Protruding-mouth cranial blood vessel ISH
Protruding-mouth trunk vasculature ISH
desma WT standard conditions Protruding-mouth cranial blood vessel ISH
Protruding-mouth trunk vasculature ISH
desmb WT standard conditions Protruding-mouth cranial blood vessel ISH
Protruding-mouth trunk vasculature ISH
EGFP y1Tg standard conditions Protruding-mouth hyaloid vessel IFL
Protruding-mouth optic artery IFL
y1Tg + MO2-foxd3 + MO3-foxd3 standard conditions Protruding-mouth hyaloid vessel IFL
Protruding-mouth optic artery IFL
pdgfrb y1Tg standard conditions Protruding-mouth hyaloid vessel pericyte ISH
Protruding-mouth optic artery pericyte ISH
y1Tg + MO2-foxd3 + MO3-foxd3 standard conditions Protruding-mouth hyaloid vessel pericyte ISH
Protruding-mouth optic artery pericyte ISH
rgs5a WT standard conditions Protruding-mouth cranial blood vessel ISH
Protruding-mouth trunk vasculature ISH
tagln2 WT standard conditions Long-pec cranial blood vessel ISH
Long-pec cranial blood vessel pericyte ISH
Long-pec intersegmental vessel ISH
Long-pec intersegmental vessel pericyte ISH
Protruding-mouth cranial blood vessel ISH
Protruding-mouth trunk vasculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development