header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-140325-14
Wang et al., 2014 - Notch3 establishes brain vascular integrity by regulating pericyte number. Development (Cambridge, England)   141(2):307-317 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 3

notch3fh332 mutant larvae have brain hemorrhage and a defective BBB. (A-D) Examples of brain hemorrhage in 3 dpf notch3fh332 mutant larvae. (A-C) Lateral view. (D) Dorsal view. Arrowheads indicate blood pooling in the brain ventricle. Insets show high magnification view of the hemorrhage in the brain ventricle. (E-H) Representative results of BBB assay in 4 dpf wild-type (WT) and mutant larvae. (E,F) Lateral views of the brain. (G,H) Dorsal views of the midbrain and hindbrain. Mutant embryos failed to retain 2000 kD Alexa488-dextran in the brain microvessels 90 minutes after the injection. Arrowheads and arrows indicate brain vessels in the wild-type and mutant larvae, respectively. (I-L) Immunostaining of 3 dpf wild-type and mutant larvae with Claudin 5 and ZO-1 antibodies. Arrowheads indicate Claudin 5 or ZO-1 staining at the blood vessels. Open arrowheads indicate ZO-1 staining along brain ventricular wall. Scale bar: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP notch3fh332/fh332; y1Tg standard conditions Protruding-mouth cranial blood vessel IFL
y1Tg standard conditions Protruding-mouth cranial blood vessel IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cldn5 IHC notch3fh332/fh332; y1Tg standard conditions Protruding-mouth cranial blood vessel
IHC y1Tg standard conditions Protruding-mouth cranial blood vessel
Ab1-tjp1 IHC notch3fh332/fh332; y1Tg standard conditions Protruding-mouth cranial blood vessel
IHC Protruding-mouth ventricular system
IHC y1Tg standard conditions Protruding-mouth cranial blood vessel
IHC Protruding-mouth ventricular system
Phenotype Details
Fish Conditions Stage Phenotype
notch3fh332/fh332 standard conditions Protruding-mouth cranial vasculature hemorrhagic, abnormal
Protruding-mouth ventricular system blood mislocalised, abnormal
Day 4 maintenance of permeability of blood-brain barrier disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development