ZFIN ID: ZDB-FIG-121205-5
Blaker-Lee et al., 2012 - Zebrafish homologs of 16p11.2, a genomic region associated with brain disorders, are active during brain development, and include two deletion dosage sensor genes. Disease models & mechanisms   5(6):834-851 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 256-cell to Long-pec

Fig. S1 16p11.2 homologs are expressed during development and in the brain. (A) RNA expression analysis by RT-PCR. Total embryo RNA was analyzed for expression of 16p11.2 homologs at 2.5 hpf (blastula), 6 hpf (early gastrula), 11 hpf (late gastrula), 18 hpf (mid-somitogenesis), 24 hpf and 48 hpf. Most genes are expressed maternally, at 2.5 hpf, and expression persists zygotically (6 hpf and later). asphd1, doc2a, prrt2 and sez6L2 show zygotic expression only, suggesting that these genes act later in development. tbx24 expression declines sharply by 48 hpf, suggesting a narrow temporal activity window. Further, this gene is not expressed in brain. (8) Spatial expression analysis by whole mount in situ hybridization. Gene expression was analyzed at 24 hpf (see Methods and Table S4). (a-t) dorsal view, (a′-t′) lateral view. All genes except tbx24 were expressed in the brain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldoaa WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
asphd1 WT standard conditions 14-19 somites to Long-pec whole organism RTPCR
Prim-5 brain ISH
cdipt WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
coro1a WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
doc2a WT standard conditions Prim-5 brain ISH
Prim-5 to Long-pec whole organism RTPCR
gdpd3a WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
hirip3 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
ino80e WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
kctd13 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
kif22 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
mapk3 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
maza WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
mvp WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
pagr1 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
ppp4ca WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
prrt2 WT standard conditions 5-9 somites to Long-pec whole organism RTPCR
sez6l2 WT standard conditions Prim-5 brain ISH
Prim-5 to Long-pec whole organism RTPCR
taok2a WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
taok2b WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
tbx6 WT standard conditions 256-cell to Prim-5 whole organism RTPCR
tlcd3ba WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
ypel3 WT standard conditions 256-cell to Long-pec whole organism RTPCR
Prim-5 brain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.