ZFIN ID: ZDB-FIG-120522-20
Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest. PLoS Genetics   8(5):e1002710 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 6

Forced expression of Id2a in CNCCs inhibits ectomesenchyme specification.

(A–F) Whole mount in situs show ectopic arch expression of sox10 at 18 hpf in sox10:Gal4VP16; UAS:Id2a embryos (n = 7/7) but not sox10:Gal4VP16 only controls (n = 0/9), loss of fli1a arch expression at 24 hpf in sox10:Gal4VP16; UAS:Id2a embryos (n = 4/4) but not sox10:Gal4VP16 only controls (n = 0/17), and mild reductions of dlx2a arch expression at 18 hpf in sox10:Gal4VP16; UAS:Id2a embryos (n = 5/5) but not sox10:Gal4VP16 only controls (n = 0/8). Arches are numbered. Arrows denote the second arch, white arrowheads the developing ear, and red arrowheads the vasculature. (G and H) Skeletal staining at 5 dpf shows severe reduction of the craniofacial skeleton in sox10:Gal4VP16; UAS:Id2a embryos (n = 17/17) compared to sox10:Gal4VP16 only controls (n = 0/22). (I and J) In situs for dct expression at 28 hpf show that melanophore precursors are unaffected in sox10:Gal4VP16; UAS:Id2a embryos (n = 8) and sox10:Gal4VP16 only controls (n = 8). (K and L) In situs for xdh expression at 28 hpf show that xanthophore precursors are unaffected in sox10:Gal4VP16; UAS:Id2a embryos (n = 4) and sox10:Gal4VP16 only controls (n = 4). (M–P) twist1a expression at 18 hpf is unaffected in sox10:Gal4VP16; UAS:Id2a embryos (n = 12) versus sox10:Gal4VP16 only controls (n = 11). twist1b expression at 18 hpf is similarly unaffected in sox10:Gal4VP16; UAS:Id2a embryos (n = 7) versus sox10:Gal4VP16 controls (n = 8). Id2a misexpression embryos are to the right in I–P. Scale bars = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aox5 el159Tg standard conditions Prim-5 xanthoblast ISH
el159Tg; el405Tg standard conditions Prim-5 xanthoblast ISH
dct el159Tg standard conditions Prim-5 melanocyte ISH
el159Tg; el405Tg standard conditions Prim-5 melanocyte ISH
dlx2a el159Tg standard conditions 14-19 somites pharyngeal arch ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
el159Tg; el405Tg standard conditions 14-19 somites pharyngeal arch ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
fli1 el159Tg standard conditions Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
Prim-5 pharyngeal arch 4 ISH
Prim-5 vasculature ISH
el159Tg; el405Tg standard conditions Prim-5 Not Detected pharyngeal arch ISH
Prim-5 vasculature ISH
sox10 el159Tg standard conditions 14-19 somites Not Detected pharyngeal arch ISH
el159Tg; el405Tg standard conditions 14-19 somites pharyngeal arch ISH
14-19 somites Not Detected pharyngeal arch 1 ISH
14-19 somites Not Detected pharyngeal arch 2 ISH
14-19 somites Not Detected pharyngeal arch 3 ISH
twist1a el159Tg standard conditions 14-19 somites pharyngeal arch ectomesenchyme ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
el159Tg; el405Tg standard conditions 14-19 somites pharyngeal arch ectomesenchyme ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
twist1b el159Tg standard conditions 14-19 somites pharyngeal arch ectomesenchyme ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
el159Tg; el405Tg standard conditions 14-19 somites pharyngeal arch ectomesenchyme ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
el159Tg; el405Tg standard conditions Day 5 splanchnocranium hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.