ZFIN ID: ZDB-FIG-120522-22
Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest. PLoS Genetics   8(5):e1002710 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 8

Misexpression of Bmp4 in migrating CNCCs inhibits ectomesenchyme formation.

(A–D) Whole mount in situs at 18 hpf show ectopic expression of sox10 in the arches (numbered) of sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 8/8) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/8) and reductions of dlx2a in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 4/4) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/4). Arrows indicate the second arch and white arrowheads the developing ear. (E–H) Double fluorescent in situs for mKR (red) and fli1a or dlx2a (green) at 24 hpf show reduction of fli1a arch expression in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 5/5) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/9) and reduction of dlx2a arch expression in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 4/4) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/3). The mKR transgene was included as a lineage tracer for CNCC-derived cells that migrated into the arches. (I,J) Skeletal staining at 5 dpf shows severe loss of craniofacial skeleton in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 7/7) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/9). (K,L) In situs for foxd3 at 48 hpf reveal largely normal patterns of glia in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 8) and sox10:Gal4VP16; UAS:mKR controls (n = 11). (M–P) In situs at 28 hpf show normal dct-positive melanophore precursors in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 12) and sox10:Gal4VP16; UAS:mKR controls (n = 15) and normal xdh-positive xanthophore precursors in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 10) and sox10:Gal4VP16; UAS:mKR controls (n = 10). Scale bars = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aox5 el159Tg; el15Tg standard conditions Prim-5 xanthoblast ISH
el159Tg; el15Tg; el49Tg standard conditions Prim-5 xanthoblast ISH
dct el159Tg; el15Tg standard conditions Prim-5 melanocyte ISH
el159Tg; el15Tg; el49Tg standard conditions Prim-5 melanocyte ISH
dlx2a el159Tg; el15Tg standard conditions 14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
Prim-5 pharyngeal arch 4 ISH
el159Tg; el15Tg; el49Tg standard conditions 14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
Prim-5 Not Detected pharyngeal arch ISH
Prim-5 Not Detected pharyngeal arch 1 ISH
Prim-5 Not Detected pharyngeal arch 2 ISH
Prim-5 Not Detected pharyngeal arch 3 ISH
fli1 el159Tg; el15Tg standard conditions Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
el159Tg; el15Tg; el49Tg standard conditions Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
foxd3 el159Tg; el15Tg standard conditions Long-pec glial cell ISH
el159Tg; el15Tg; el49Tg standard conditions Long-pec glial cell ISH
KillerRed el159Tg; el15Tg standard conditions Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
Prim-5 pharyngeal arch 4 ISH
el159Tg; el15Tg; el49Tg standard conditions Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 1 ISH
Prim-5 pharyngeal arch 2 ISH
Prim-5 pharyngeal arch 3 ISH
sox10 el159Tg; el15Tg standard conditions 14-19 somites otic vesicle ISH
14-19 somites Not Detected pharyngeal arch ISH
el159Tg; el15Tg; el49Tg standard conditions 14-19 somites otic vesicle ISH
14-19 somites pharyngeal arch ISH
14-19 somites pharyngeal arch 1 ISH
14-19 somites pharyngeal arch 2 ISH
14-19 somites pharyngeal arch 3 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
el159Tg; el15Tg; el49Tg standard conditions Day 5 splanchnocranium hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.