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ZFIN ID: ZDB-FIG-110224-14
Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures. Developmental Biology   350(2):484-495 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to Prim-5

Fig. 8 Flh and FoxAs transcription factors are both essential for the maintenance of axial structures. (A–H) Expression at 24 hpf of the notochord marker im:7144261 (A–D) and the floor plate and hypochord marker col2a1a (E–H). Embryos injected with low dose of MO-Flh (0.8 ng) (B and F) or MO-FoxA2–FoxA3 (3 x 4 ng) (C and G) display weak phenotypes compared to wild-type (A and E). With the same amount of morpholinos, the triple inactivation of Flh, FoxA2 and FoxA3 completely abolishes notochord (D), floor plate and hypochord (H) development. (I–P) Expression at 80% epiboly of the chordamesoderm marker twist2 (I–L) and paraxial mesoderm marker myf5 (M–P). Partial Flh (J and N) or FoxA2–FoxA3 (K and O) morphants show a slight reduction of axial mesoderm. Partial Flh-FoxA2–FoxA3 triple morphants lack all axial mesoderm (L) that is accompanied by a complete fusion of paraxial mesoderm along the dorsal midline (P). (Q–T) Expression of flh at the shield stage in strong Flh (R), FoxA2–FoxA3 (S) and Flh-FoxA2–FoxA3 (T) morphants is similar to wild-type embryo (Q). (A–H) Lateral views, anterior to the left. (I–P) Dorsal views, anterior up. (Q–T) Lateral views, dorsal to the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
col2a1a WT control Prim-5 floor plate ISH
Prim-5 hypochord ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Prim-5 floor plate ISH
Prim-5 hypochord ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions Prim-5 Not Detected floor plate ISH
Prim-5 Not Detected hypochord ISH
WT + MO2-noto standard conditions Prim-5 floor plate ISH
Prim-5 hypochord ISH
im:7144261 WT control Prim-5 notochord ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Prim-5 notochord ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions Prim-5 Not Detected notochord ISH
WT + MO2-noto standard conditions Prim-5 notochord ISH
myf5 WT control 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO2-noto standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
noto WT control Shield shield ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Shield shield ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions Shield shield ISH
WT + MO2-noto standard conditions Shield shield ISH
twist2 WT control 75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions 75%-epiboly Not Detected axial chorda mesoderm ISH
WT + MO2-noto standard conditions 75%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Shield shield morphology, normal
75%-epiboly axial chorda mesoderm decreased width, abnormal
75%-epiboly paraxial mesoderm increased area, abnormal
Prim-5 floor plate disorganized, abnormal
Prim-5 hypochord disorganized, abnormal
Prim-5 notochord disorganized, abnormal
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 + MO2-noto standard conditions Shield shield morphology, normal
75%-epiboly axial chorda mesoderm aplastic, abnormal
75%-epiboly paraxial mesoderm increased area, abnormal
75%-epiboly paraxial mesoderm left side fused with paraxial mesoderm right side, abnormal
Prim-5 floor plate aplastic, abnormal
Prim-5 hypochord aplastic, abnormal
Prim-5 notochord aplastic, abnormal
WT + MO2-noto standard conditions Shield shield morphology, normal
75%-epiboly axial chorda mesoderm decreased width, abnormal
75%-epiboly paraxial mesoderm increased area, abnormal
Prim-5 floor plate disorganized, abnormal
Prim-5 hypochord disorganized, abnormal
Prim-5 notochord disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.