ZFIN ID: ZDB-FIG-110224-19
Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures. Developmental Biology   350(2):484-495 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Prim-5

Fig. S5 Phenotype of floating head morphant. (A and B) Embryos injected with 1.6 ng MO-Flh do not develop notochord and hypochord and present a patchy floor plate and fused somites at 24 hpf. (B) High magnification of the trunk region of Flh morphants. (C–F) Analysis of twist2 (C and D) and myf5 (E and F) expression at 80% epiboly. In Flh morphants, twist2 expression domain is narrower (D) and myf5 territory is partially fused along the dorsal midline (F). (G–J) At shield stage, expressions of foxA2 (G and H) and foxA3 (I and J) are not affected in Flh morphants (H and J) compared to wild-type (G and I). (A and B) Lateral views, anterior to the left. (C–F) Dorsal views, anterior up. (G–J) Animal pole views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxa2 WT control Shield hypoblast ISH
WT + MO2-noto standard conditions Shield hypoblast ISH
foxa3 WT control Shield hypoblast ISH
WT + MO2-noto standard conditions Shield hypoblast ISH
myf5 WT control 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO2-noto standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
twist2 WT control 75%-epiboly axial chorda mesoderm ISH
WT + MO2-noto standard conditions 75%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-noto standard conditions Shield shield morphology, normal
WT + MO2-noto standard conditions 75%-epiboly axial chorda mesoderm decreased width, abnormal
WT + MO2-noto standard conditions 75%-epiboly paraxial mesoderm increased area, abnormal
WT + MO2-noto standard conditions 75%-epiboly paraxial mesoderm left side fused with paraxial mesoderm right side, abnormal
WT + MO2-noto standard conditions Prim-5 floor plate disorganized, abnormal
WT + MO2-noto standard conditions Prim-5 hypochord aplastic, abnormal
WT + MO2-noto standard conditions Prim-5 notochord aplastic, abnormal
WT + MO2-noto standard conditions Prim-5 somite fused with somite, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.