ZFIN ID: ZDB-FIG-110224-9
Dal-Pra et al., 2011 - FoxA transcription factors are essential for the development of dorsal axial structures. Developmental Biology   350(2):484-495 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 3 FoxA2 and FoxA3 are required for the maintenance but not for the induction of axial mesoderm. (A–J) Expression at 80% epiboly of the chordamesoderm marker twist2 (A–E) and the paraxial mesoderm marker myf5 (F–J) in FoxA2 (B and G) or FoxA3 (C and H) and FoxA2–FoxA3 (D, E, I, and J) morphants. twist2 and myf5 expressions are identical to wild-type (A and F) in FoxA2 morphants or slightly affected in FoxA3 morphants. In double FoxA2–FoxA3 morphants, axial mesoderm is narrower (D) or almost absent (E), while paraxial mesoderm is dorsally extended (I) or partially fused along the dorsal midline (J). (K–N) flh expression domain in FoxA2–FoxA3 morphants is not affected at the shield stage (L) and is narrower at 80% epiboly (N) compared to wild-type (K and M). Dorsal views, anterior up except (K and L) lateral views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myf5 WT control 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxa2 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
noto WT control Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
twist2 WT control 75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa2 standard conditions 75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm ISH
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa2 standard conditions 75%-epiboly axial chorda mesoderm morphology, normal
75%-epiboly paraxial mesoderm morphology, normal
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions Shield shield morphology, normal
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm aplastic, abnormal
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm decreased width, abnormal
WT + MO1-foxa2 + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly paraxial mesoderm increased area, abnormal
WT + MO1-foxa3 + MO2-foxa3 standard conditions 75%-epiboly axial chorda mesoderm decreased width, abnormal
75%-epiboly paraxial mesoderm increased area, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 350(2), Dal-Pra, S., Thisse, C., and Thisse, B., FoxA transcription factors are essential for the development of dorsal axial structures, 484-495, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.