Eve1 overexpression causes anterior truncation, suppression of anterior markers, and induction of posterior markers. Zebrafish embryos were injected with eve1 mRNA (as indicated at the bottom left corner of each panel; Ctrl, uninjected controls). (A–C) Eve1 mRNA injected embryos at 48 hpf showing anterior truncation and progressive loss of posterior patterning. (D–O) In situ hybridization of control and eve1 mRNA-injected embryos at 80% epiboly (lateral views, dorsal to the right, where discernible). Genes analyzed are indicated in the top right corner. Expression of the anterior gene otx2 and epidermal gene p63 was suppressed (D, E; N, O), whereas the expression of hoxb1b was expanded by eve1 overexpression (F, G). Expansion also was observed for sox3, zic2a, and sox31 (H–M).