ZFIN ID: ZDB-FIG-101123-56
Cruz et al., 2010 - Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos. Proc. Natl. Acad. Sci. USA   107(8):3564-3569 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 75%-epiboly

Fig. 1

Eve1 overexpression causes anterior truncation, suppression of anterior markers, and induction of posterior markers. Zebrafish embryos were injected with eve1 mRNA (as indicated at the bottom left corner of each panel; Ctrl, uninjected controls). (AC) Eve1 mRNA injected embryos at 48 hpf showing anterior truncation and progressive loss of posterior patterning. (DO) In situ hybridization of control and eve1 mRNA-injected embryos at 80% epiboly (lateral views, dorsal to the right, where discernible). Genes analyzed are indicated in the top right corner. Expression of the anterior gene otx2 and epidermal gene p63 was suppressed (D, E; N, O), whereas the expression of hoxb1b was expanded by eve1 overexpression (F, G). Expansion also was observed for sox3, zic2a, and sox31 (HM).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hoxb1b WT control 75%-epiboly whole organism ISH
otx2b WT control 75%-epiboly anterior presumptive neural plate ISH
sox3 WT control 75%-epiboly presumptive neural plate ISH
sox19b WT control 75%-epiboly presumptive neural plate ISH
tp63 WT control 75%-epiboly ectoderm ISH
zic2a WT control 75%-epiboly presumptive neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA