ZFIN ID: ZDB-FIG-101123-57
Cruz et al., 2010 - Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America   107(8):3564-3569 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 75%-epiboly

Fig. 2

Eve1 depletion suppresses trunk and tail development, and eve1 acts as a repressor. Zebrafish embryos were injected with eve1MO (B, C, H, L, P, T) or with eve1-VP16 mRNA (E, F, I, M, Q, U) as shown at bottom right of panels B, C, E, and F and at the top of the columns for the remainder. Embryos at 24 hpf (AC) and at 28 hpf (DF), show variable loss of trunk and tail tissue. (GV) In situ staining of embryos at 70–80% epiboly (GR) and 60% epiboly (SV): lateral views, dorsal to the right (where discernible), with probes shown at the left of the rows. Hoxb1b and aldh1a2 are suppressed by eve1MO (H and L) and eve1-VP16 (I and M, compare with G and K), and expression of both genes is expanded in embryos injected with eve1-Eng (J and N). Conversely, bmp2b and bmp4 expression domains are expanded in embryos injected with eve1MO (P and T) and eve1-VP16 (Q and U, compare with O and S), whereas eve1-Eng suppresses expression of both BMPs (R and V).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aldh1a2 WT control 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-eve1 standard conditions 75%-epiboly Not Detected presumptive paraxial mesoderm ISH
bmp2b WT control 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
WT + MO1-eve1 standard conditions 75%-epiboly ectoderm ISH
75%-epiboly margin ISH
bmp4 WT control Shield unspecified ISH
WT + MO1-eve1 standard conditions Shield whole organism ISH
hoxb1b WT control 75%-epiboly presumptive neural plate ISH
WT + MO1-eve1 standard conditions 75%-epiboly Not Detected presumptive neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA