ZFIN ID: ZDB-FIG-101123-58
Cruz et al., 2010 - Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America   107(8):3564-3569 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 75%-epiboly

Fig. 3 Eve1 induces hoxb1b expression via an RA signal. (AJ and MS) Dorsal and (K and L) lateral views (where discernible, dorsal to the right) of zebrafish embryos fixed for in situ staining at 80% epiboly (AL) and 60% epiboly (MS) Injections are indicated at the bottom left of each panel, and genes analyzed are given at the top right. (AD) Suppression of otx2 by eve1 does not depend on RA because it resists overexpression of the RA-metabolizing enzyme Cyp26a1. (EH) Eve1-mediated induction of hoxb1b does not occur when eve1 and cyp26a1 mRNAs are coinjected (G), and cyp26a1 injection alone suppresses hoxb1b expression (H; only one of two cells was injected in this embryo). (I and J) Eve1 induces aldh1a2 expression. (K and L) Anterior expression of cyp26a1 is suppressed by eve1 but remains unaffected at the margin. (MO) Injection of high concentrations of aldMO and eve1 mRNA (Materials and Methods). Eve1 cannot rescue hoxb1b expression in aldMO-injected embryos (O). (PS) Injection of low concentrations of eve1MO (2 ng/nL) and aldMO showed synergism in the suppression of hoxb1b.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 WT control 75%-epiboly presumptive paraxial mesoderm ISH
cyp26a1 WT control 75%-epiboly neuroectoderm ISH
hoxb1b WT control 75%-epiboly presumptive neural plate ISH
WT + MO1-eve1 standard conditions 75%-epiboly presumptive neural plate ISH
WT + MO5-aldh1a2 standard conditions 75%-epiboly presumptive neural plate ISH
otx2b WT control 75%-epiboly anterior presumptive neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA