header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-101123-60
Cruz et al., 2010 - Induction and patterning of trunk and tail neural ectoderm by the homeobox gene eve1 in zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America   107(8):3564-3569 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5

Eve1 rescues posterior neural development in ich-/- mutants. Homozygous ich-/- embryos were injected with eve1 mRNA. Genotype is indicated at the bottom left of each panel, injections at the bottom right, and in situ probes at the top right. Uninjected ich-/- embryos at 24 hpf (A). Eve1 mRNA injection leads to varying levels of rescue of posterior dorsal axis (C–E). Embryos stained for hoxb1b at 80% epiboly, presumed lateral view, dorsal to the right. Expression of hoxb1b is absent in uninjected ich-/- embryos (C, D) but is rescued by injection of eve1 mRNA (E). In situ hybridization of wild-type and ich-/- embryos at 24 hpf (anterior to the left) (F-Q). Neural gene expression and posterior dorsal axis formation was partially rescued by the injection of eve1 mRNA. Rescue of pax2a appears to extend to the midbrain–hindbrain boundary (L-N, arrowheads), whereas egr2b expression appears to extend to rhombomere 5 (OQ, arrowheads).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
ctnnb2p1/p1 standard conditions Prim-5 Not Detected rhombomere 3 ISH
Prim-5 Not Detected rhombomere 5 ISH
elavl3 WT standard conditions Prim-5 central nervous system ISH
ctnnb2p1/p1 standard conditions Prim-5 Not Detected central nervous system ISH
hoxb1b WT standard conditions 75%-epiboly presumptive neural plate ISH
ctnnb2p1/p1 standard conditions 75%-epiboly Not Detected presumptive neural plate ISH
pax2a WT standard conditions Prim-5 midbrain hindbrain boundary ISH
ctnnb2p1/p1 standard conditions Prim-5 Not Detected midbrain hindbrain boundary ISH
sox3 WT control Prim-5 brain ISH
Prim-5 spinal cord ISH
ctnnb2p1/p1 standard conditions Prim-5 Not Detected brain ISH
Prim-5 Not Detected spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ctnnb2p1/p1 standard conditions Prim-5 head aplastic, abnormal
Prim-5 trunk morphology, abnormal
Prim-5 whole organism wholly ventralized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA