ZFIN ID: ZDB-FIG-091113-116
Sanek et al., 2009 - Zebrafish zic2a patterns the forebrain through modulation of Hedgehog-activated gene expression. Development (Cambridge, England)   136(22):3791-3800 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites

Fig. 7 Patterned gene expression in OS and retinal precursors. (A-F) Wild-type embryos at 16s stained using wholemount in situ hybridization (WISH, green) for expression of pax2a (A), pax6a (B), fgf8a (C), zic2a (D) and vax1 (E). Nuclei were counterstained using DAPI (red). The schematic (F) illustrates the overlap between zic2a and pax2a expression domains in the presumptive OS, and the overlap between pax2a and pax6a domains in the presumptive retina. (G-I) Expression of zic2a (G), pax2a (H) and pax6a (I) detected at 23s. Expression patterns, imaged using confocal microscopy, are shown as z-stacks in ventral view, anterior up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fgf8a AB standard conditions 14-19 somites forebrain ISH
pax2a AB standard conditions 14-19 somites optic stalk ISH
14-19 somites optic vesicle ISH
20-25 somites optic stalk ISH
pax6a AB standard conditions 14-19 somites optic vesicle ISH
20-25 somites optic cup ISH
vax1 AB standard conditions 14-19 somites optic stalk ISH
14-19 somites optic vesicle ISH
zic2a AB standard conditions 14-19 somites optic stalk ISH
14-19 somites optic vesicle ISH
20-25 somites optic stalk ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development