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ZFIN ID: ZDB-FIG-090113-7
Sabel et al., 2009 - Maternal Interferon Regulatory Factor 6 is required for the differentiation of primary superficial epithelia in Danio and Xenopus embryos. Developmental Biology   325(1):249-262 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Shield

Fig. 3 Irf6DBD-mediated changes in gene expression in shield-stage D. rerio embryos. Dorsal views, except as indicated, of uninjected and irf6DBD-injected embryos, as indicated by the column header, fixed when uninjected sibling embryos reached shield stage. Embryos processed for in situ hybridization to reveal expression of the indicated gene, except as noted. Images portray the phenotype displayed by 60%–80% of embryos in the experimental group, n = 25 embryos or greater. (A–F) Expression of zfk8, krt18, and cebpb, are expressed in all EVL cells of uninjected embryos but absent in large patches in uninjected embryos. (G) Uninjected and, (H) irf6DBD-injected embryos stained with phalloidin, revealing polymerized actin (i.e., F-actin). (G) In the uninjected embryo, F-actin is visible in a cortical ring within every EVL cell. (H) In the irf6DBD-injected embryo, there is loss (*) or disorganization of F-actin in superficial cells. (I, J) Lateral views, showing gata2 expression in non-neural epiblast is present but displaced the irf6DBD-injected embryo. (K, L) In contrast to gata2, expression of tfap2a, a marker of non-neural epiblast, is highly reduced in the irf6DBD-injected embryo. (M,N) sox2 expression, a marker of neural epiblast, is present in the irf6DBD-injected embryo. (O, P) Lateral views, showing that ntl expression, a marker of hypoblast, is present in the irf6DBD-injected embryo. (Q, R) gsc expression is present in the shield hypoblast of the uninjected embryo, and in an expanded arc in the irf6DBD-injected embryo, consistent with developmental delay in the latter (arrowheads show extent of gsc expression arc). (S, T) Lateral views of embryos processed to reveal cells undergoing apoptosis by TUNEL. TUNEL-positive cells are absent from this uninjected embryo. In the irf6DBD-injected embryo, many TUNEL-positive cells are visible, primarily within the EVL.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebpb WT standard conditions Shield EVL ISH
WT chemical treatment: drug Shield EVL ISH
gata2a WT standard conditions Shield epiblast non neural ectoderm ISH
WT chemical treatment: drug Shield epiblast non neural ectoderm ISH
gsc WT standard conditions Shield shield hypoblast ISH
WT chemical treatment: drug Shield shield hypoblast ISH
krt8 WT standard conditions Shield EVL ISH
WT chemical treatment: drug Shield EVL ISH
krt18a.1 WT standard conditions Shield EVL ISH
WT chemical treatment: drug Shield EVL ISH
sox2 WT standard conditions Shield epiblast neuroectoderm ISH
WT chemical treatment: drug Shield epiblast neuroectoderm ISH
tbxta WT standard conditions Shield hypoblast ISH
WT chemical treatment: drug Shield hypoblast ISH
tfap2a WT standard conditions Shield epiblast non neural ectoderm ISH
WT chemical treatment: drug Shield epiblast non neural ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: drug Shield apoptotic process increased occurrence, abnormal
Shield epithelium development process quality, abnormal
Shield EVL disorganized, abnormal
Shield gastrulation with mouth forming second delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 325(1), Sabel, J.L., d'Alençon, C., O'Brien, E.K., Otterloo, E.V., Lutz, K., Cuykendall, T.N., Schutte, B.C., Houston, D.W., and Cornell, R.A., Maternal Interferon Regulatory Factor 6 is required for the differentiation of primary superficial epithelia in Danio and Xenopus embryos, 249-262, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.