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ZFIN ID: ZDB-FIG-080715-28
Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal. Developmental Biology   319(2):192-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3 Expression of Prl, Gh, and Pit-1 in FGF3 morphants are intact. In situ hybridization at 56 hpf using probes indicated in the lower right corner of each panel. (A–D) Wild type embryos. (E–H) Low dose FGF3 morphants with diminished POMC-GFP expression in the pars intermedia. (I–L) High dose FGF3 morphants with diminished POMC-GFP expression in the pars intermedia and rostral pars distalis. Ventral view of head, anterior to the left. Arrowheads, RPD. Arrow, PI. P, pituitary; Hy, hypothalamic POMC cells. Scale bar, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gh1 WT standard conditions Long-pec adenohypophyseal placode ISH
zf44Tg + MO1-fgf3 standard conditions Long-pec adenohypophyseal placode ISH
pomca WT standard conditions Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec pars anterior ISH
Long-pec pars intermedia ISH
zf44Tg + MO1-fgf3 standard conditions Long-pec hypothalamus ISH
Long-pec pars anterior ISH
Long-pec Not Detected pars intermedia ISH
pou1f1 WT standard conditions Long-pec adenohypophyseal placode ISH
zf44Tg + MO1-fgf3 standard conditions Long-pec adenohypophyseal placode ISH
prl WT standard conditions Long-pec adenohypophyseal placode ISH
zf44Tg + MO1-fgf3 standard conditions Long-pec adenohypophyseal placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf44Tg + MO1-fgf3 standard conditions Long-pec adenohypophysis morphogenesis process quality, abnormal
Long-pec pars intermedia physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.