ZFIN ID: ZDB-FIG-080715-26
Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal. Developmental Biology   319(2):192-200 Full text @ Dev. Biol.
Anatomical Term:
Stage Range: Prim-5 to Prim-25
Observed In:
Stage: Prim-25

Fig. 1 Temporally and spatially distinct POMC cell ontogeny in zebrafish pituitary. (A–F) In vivo time-lapse imaging of POMC-GFP expressing-cells in the pituitary anlage visualized using a Leica TCS SP confocal microscope from 28 to 42 hpf. Developmental stages are indicated at bottom right corner. POMC-GFP expressing cells are detected within the anterior most and lateral-posterior surface of ANR at 28 hpf (A), followed by asymmetric bilateral-posterior POMC cell differentiation and medio-posterior migration into the rostral pars distalis (RPD) and pars intermedia (PI). (G and H) in situ hybridization at 32 hpf using a POMC probe. Note the endogenous pituitary pomc expression pattern is similar to that of POMC-GFP transgene. (I–L) Treatment of POMC-GFP transgenic embryo with 10 μM SU5402 or DMSO between 22–26 hpf leads to diminished POMC-GFP cells in PI by 36 hpf visualized under a fluorescein isothiocyanate (FITC) filter on a Zeiss microscope. (I and K), DIC images showing pituitary anlage outlined by dashed arrows. (J and L), POMC-GFP fluorescent images superimposed onto DIC images. A–F and I–L: ventral view; G: ventral–frontal view; H: ventrolateral view (top, dorsal; bottom, ventral). All: left, anterior; right, posterior. Arrowhead: anterior-most or RPD POMC cells; arrow: lateral-posterior or PI POMC cells; Hy: hypothalamic POMC expressing cells. The anterior-most neural ridge surface is traced as a reference point in panel A. e, eye. Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf44Tg control Prim-25 adenohypophyseal placode IFL
zf44Tg standard conditions Prim-5 adenohypophyseal placode IFL
Prim-15 adenohypophyseal placode IFL
Prim-25 adenohypophyseal placode IFL
zf44Tg chemical treatment: SU5402 Prim-25 adenohypophyseal placode IFL
pomca WT standard conditions Prim-15 adenohypophyseal placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf44Tg chemical treatment: SU5402 Prim-25 adenohypophysis morphogenesis process quality, abnormal
Prim-25 pars intermedia physical object quality, abnormal
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Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.