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ZFIN ID: ZDB-FIG-080715-26
Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal. Developmental Biology   319(2):192-200 Full text @ Dev. Biol.
Anatomical Term:
Stage Range: Prim-5 to Prim-25
Observed In:
Stage: Prim-25

Fig. 1 Temporally and spatially distinct POMC cell ontogeny in zebrafish pituitary. (A–F) In vivo time-lapse imaging of POMC-GFP expressing-cells in the pituitary anlage visualized using a Leica TCS SP confocal microscope from 28 to 42 hpf. Developmental stages are indicated at bottom right corner. POMC-GFP expressing cells are detected within the anterior most and lateral-posterior surface of ANR at 28 hpf (A), followed by asymmetric bilateral-posterior POMC cell differentiation and medio-posterior migration into the rostral pars distalis (RPD) and pars intermedia (PI). (G and H) in situ hybridization at 32 hpf using a POMC probe. Note the endogenous pituitary pomc expression pattern is similar to that of POMC-GFP transgene. (I–L) Treatment of POMC-GFP transgenic embryo with 10 μM SU5402 or DMSO between 22–26 hpf leads to diminished POMC-GFP cells in PI by 36 hpf visualized under a fluorescein isothiocyanate (FITC) filter on a Zeiss microscope. (I and K), DIC images showing pituitary anlage outlined by dashed arrows. (J and L), POMC-GFP fluorescent images superimposed onto DIC images. A–F and I–L: ventral view; G: ventral–frontal view; H: ventrolateral view (top, dorsal; bottom, ventral). All: left, anterior; right, posterior. Arrowhead: anterior-most or RPD POMC cells; arrow: lateral-posterior or PI POMC cells; Hy: hypothalamic POMC expressing cells. The anterior-most neural ridge surface is traced as a reference point in panel A. e, eye. Scale bars, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf44Tg control Prim-25 adenohypophyseal placode IFL
zf44Tg standard conditions Prim-5 adenohypophyseal placode IFL
Prim-15 adenohypophyseal placode IFL
Prim-25 adenohypophyseal placode IFL
zf44Tg chemical treatment: SU5402 Prim-25 adenohypophyseal placode IFL
pomca WT standard conditions Prim-15 adenohypophyseal placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf44Tg chemical treatment: SU5402 Prim-25 adenohypophysis morphogenesis process quality, abnormal
Prim-25 pars intermedia physical object quality, abnormal
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.