ZFIN ID: ZDB-FIG-080715-29
Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal. Developmental Biology   319(2):192-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4 Lineage specific suppression of PI POMC cells in FGF3 morphants. 1–2 cell stage POMC-GFP/PRL-RFP double transgenic embryos were either injected with 0.4 ng of FGF3 MO (B) or vehicle (A) and then cultured in routine medium. At 56 hpf, pituitary POMC-GFP/PRL-RFP expression patterns were analyzed in live embryos using confocal microscopy. In FGF3 morphant, POMC-GFP expressing cells are detected within the rostral pars distalis but not in the pars intermedia, whereas PRL-RFP expression is intact. Ventral pituitary view under high magnification (63x), anterior to the left. WT, wild type; MO, FGF3 morphant. RPD, rostral pars distalis; PI, pars intermedia. Scale bar, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP zf113Tg; zf44Tg control Long-pec pars anterior IFL
Long-pec pars intermedia IFL
zf113Tg; zf44Tg + MO1-fgf3 standard conditions Long-pec pars anterior IFL
Long-pec Not Detected pars intermedia IFL
RFP zf113Tg; zf44Tg control Long-pec pars anterior IFL
zf113Tg; zf44Tg + MO1-fgf3 standard conditions Long-pec pars anterior IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf113Tg; zf44Tg + MO1-fgf3 standard conditions Long-pec adenohypophysis morphogenesis process quality, abnormal
Long-pec pars intermedia physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.