ZFIN ID: ZDB-FIG-070314-44
Liu et al., 2007 - Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros. Development (Cambridge, England)   134(6):1111-1122 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 5 jagged 2 loss of function transfates pronephric cells to MCCs. Three markers of MCCs - shippo1 (A-C), flr (D-F) and rfx2 (G-I) - show expanded expression in the pronephros of jagged 2 morphants at 34 hpf. Control invert morpholino (A,D,G) had no effect on MCC-marker expression, whereas ATG blocking (J2atgMO; B,E,H) and exon 20 mis-splicing morpholinos (J2ex20MO; C,F,I) resulted in uniform expression of MCC markers. (J) MCCs exist as single cells (arrowheads; flr-expressing cells) in the wild-type pronephros in cross section. (K,L) Expressions of shippo1 (K) and flr (L) expand to include all cells in tubule cross sections in J2exon20 morphant embryos. (M-O) slc13a1 expression seen in the control (M) is lost in J2atg (N) and J2exon20 (O) morphants. Similarly, the observed expression of trpM7 in control morpholino-injected embryos (P) is lost in J2exon20 morphants (Q). Control jagged 2 morpholino does not alter the single-cell jagged 2 mRNA expression pattern (R), whereas combined injection of both jagged 2 atg and exon 20 antisense morpholinos (U) result in expanded jagged 2 mRNA expression. (S) Na,K-ATPase:GFP transgenic zebrafish express GFP in the pronephros distal segments (arrowheads). (T) GFP expression is lost specifically in the distal segment of jagged 2 exon 20 morphant embryos (arrowheads).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
flr WT control Prim-15 pronephros ISH
WT + MO1-jag2b standard conditions Prim-15 pronephros ISH
WT + MO4-jag2b standard conditions Prim-15 pronephros ISH
jag2b WT control Prim-15 pronephros ISH
WT + MO1-jag2b + MO4-jag2b standard conditions Prim-15 pronephros ISH
odf3b WT control Prim-15 pronephros ISH
WT + MO1-jag2b standard conditions Prim-15 pronephros ISH
WT + MO4-jag2b standard conditions Prim-15 pronephros ISH
rfx2 WT control Prim-15 pronephros ISH
WT + MO1-jag2b standard conditions Prim-15 pronephros ISH
WT + MO4-jag2b standard conditions Prim-15 pronephros ISH
slc13a1 WT control Prim-15 pronephros ISH
WT + MO1-jag2b standard conditions Prim-15 Not Detected pronephros ISH
WT + MO4-jag2b standard conditions Prim-15 Not Detected pronephros ISH
trpm7 WT control Prim-15 pronephros ISH
WT + MO4-jag2b standard conditions Prim-15 Not Detected pronephros ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-jag2b standard conditions Prim-15 cell differentiation involved in pronephros development disrupted, abnormal
Prim-15 pronephros multi-ciliated epithelial cell increased amount, abnormal
WT + MO4-jag2b standard conditions Prim-15 cell differentiation involved in pronephros development disrupted, abnormal
Prim-15 pronephros multi-ciliated epithelial cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development