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ZFIN ID: ZDB-FIG-050209-9
Karlstrom et al., 2003 - Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development. Development (Cambridge, England)   130(8):1549-1564 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 20-25 somites

Fig. 8 gli2 MO injection rescues nk2.2 and myoD expression defects in yot/gli2 mutants and reveals a weak activator role for Gli2. (A) gli2 MO injection expands ptc1 expression ventrally in the diencephalon (arrowheads) and causes a minor but consistent overall expansion of ptc1 expression (compare to inset). (B) yot/gli2 mutants have significantly reduced ptc1 expression. (C) gli2MO injections rescue the ptc1 defects seen in yot/gli2 mutants and expand ptc1 expression ventrally (arrowhead). (D) Injection of gli2MOs into wild-type embryos has no effect on nk2.2 expression. (E,F) Injection of a gli2MOs into yot–/–mutant embryos can completely rescue yot-induced defects in nk2.2 expression (compare arrows). (G) gli2MO injection does not affect myoD expression in adaxial cells (arrowheads). (H,I) gli2MO injections partially rescue yot-induced defects in adaxial myoD expression (compare arrowheads). (J-M) Injection of 3-10 ng of gli2MO into embryos from a cross between dtr–/+heterozygous parents (25% dtr–/–mutants expected) resulted in an additional loss of nk2.2 expression in the tegmentum (compare arrows in J,K) and a reduction in adaxial myoD expression (compare arrowheads in L,M) in 60/206 embryos (29%), all of which were dtr–/–mutants as judged by forebrain and hindbrain nk2.2 expression defects. This suggests Gli2 may activate Hh signaling in a small area of the ventral midbrain and in adaxial cells. Control MO injections had no effect on nk2.2 expression in 85/85 embryos from a similar dtr–/+x dtr–/+cross, with 25 embryos (29%) showing the dtr–/–nk2.2 defects (J) and 60 embryos (71%) showing wild-type nk2.2 expression as expected for dtr–/+ and dtr+/+embryos. (A-F,J, and K) are lateral views of the head, eyes removed. (G-I,L, and M) are dorsal views of the tail region. All embryos are at the 20 somite (19 hour) stage. For yot/gli2, embryo genotypes were inferred by myoD expression in adaxial cells, then were verified by PCR (not shown, see Materials and Methods). D and G, E and H, F and I, J and L and K and M show the same individual labeled simultaneously with nk2.2 and myoD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions 20-25 somites adaxial cell ISH
20-25 somites somite ISH
WT + MO1-gli2a standard conditions 20-25 somites adaxial cell ISH
20-25 somites somite ISH
gli1ts269/ts269 standard conditions 20-25 somites adaxial cell ISH
20-25 somites somite ISH
gli1ts269/ts269 + MO1-gli2a standard conditions 20-25 somites adaxial cell ISH
20-25 somites somite ISH
gli2aty119/ty119 standard conditions 20-25 somites somite ISH
gli2aty119/ty119 + MO1-gli2a standard conditions 20-25 somites adaxial cell ISH
20-25 somites somite ISH
nkx2.2a WT + MO1-gli2a standard conditions 20-25 somites central nervous system ventral region ISH
gli1ts269/ts269 standard conditions 20-25 somites forebrain ventral region ISH
20-25 somites midbrain ISH
gli1ts269/ts269 + MO1-gli2a standard conditions 20-25 somites forebrain ventral region ISH
20-25 somites Not Detected midbrain ISH
gli2aty119/ty119 standard conditions 20-25 somites central nervous system ventral region ISH
gli2aty119/ty119 + MO1-gli2a standard conditions 20-25 somites central nervous system ventral region ISH
ptch2 WT standard conditions 20-25 somites central nervous system ventral region ISH
WT + MO1-gli2a standard conditions 20-25 somites central nervous system ventral region ISH
20-25 somites diencephalon ISH
gli2aty119/ty119 standard conditions 20-25 somites central nervous system ventral region ISH
gli2aty119/ty119 + MO1-gli2a standard conditions 20-25 somites central nervous system ventral region ISH
20-25 somites diencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development