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ZFIN ID: ZDB-FIG-050209-4
Karlstrom et al., 2003 - Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development. Development (Cambridge, England)   130(8):1549-1564 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 1 dtr–/–, yot–/–and dtr–/+;yot–/+embryos have defects in body axis formation and expression of Hh target genes in the brain. (A-D) Examination of live 36-hour embryos reveals curled body axes in dtr–/–, yot–/–and dtr–/+;yot–/+mutant embryos. U-shaped somites, indicative of defects in slow muscle cell differentiation, are seen only in yot–/–and dtr–/+;yot–/+embryos, dtr–/–embryos have wild-type somites (insets). (E-H) patched 1 (ptc1) expression is generally reduced in all three genotypes. In situ labeling was performed simultaneously and embryos were developed for the same amount of time in E, F and G. Inset in H shows wild-type sibling developed in same tube as this transheterozygote. (I-L) In all gli mutant embryos, nk2.2 expression is reduced or absent from the anterior pituitary anlage (arrowheads), as well as from different regions of the ventral midbrain and ventral hindbrain. (M-P) Expression of pax6, a gene known to be repressed by Hh signaling, is variably expanded in the MDB (arrowhead) and hindbrain (arrows). Expression of pax6 is expanded across the MDB expression domain of shh (not shown), ptc (E), and nk2.2 (I). All panels show lateral views, anterior to the left. Eyes were removed in E-P. Gene expression is indicated on the left. Di, diencephalon; HB, hindbrain; MB, midbrain; MDB, mid-diencephalon boundary; MHB, midbrain-hindbrain boundary; te, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.2a WT standard conditions Prim-25 adenohypophyseal placode ISH
Prim-25 diencephalon ISH
Prim-25 forebrain ISH
Prim-25 hindbrain ventral region ISH
Prim-25 midbrain ISH
Prim-25 midbrain ventral region ISH
Prim-25 neuroectoderm ventral region ISH
Prim-25 spinal cord ISH
gli1ts269/+; gli2aty119/+ standard conditions Prim-25 adenohypophyseal placode ISH
Prim-25 central nervous system ventral region ISH
Prim-25 forebrain ventral region ISH
Prim-25 midbrain ISH
Prim-25 Not Detected spinal cord ISH
gli1ts269/ts269 standard conditions Prim-25 adenohypophyseal placode ISH
Prim-25 central nervous system ventral region ISH
Prim-25 forebrain ventral region ISH
Prim-25 midbrain ISH
Prim-25 Not Detected spinal cord ISH
gli2aty119/ty119 standard conditions Prim-25 adenohypophyseal placode ISH
Prim-25 central nervous system ventral region ISH
Prim-25 forebrain ventral region ISH
Prim-25 midbrain ISH
Prim-25 Not Detected spinal cord ISH
pax6a WT standard conditions Long-pec forebrain ISH
Long-pec forebrain midbrain boundary ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
gli1ts269/+; gli2aty119/+ standard conditions Long-pec forebrain ISH
Long-pec forebrain midbrain boundary ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
gli1ts269/ts269 standard conditions Long-pec forebrain ISH
Long-pec forebrain midbrain boundary ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
gli2aty119/ty119 standard conditions Long-pec forebrain ISH
Long-pec forebrain midbrain boundary ISH
Long-pec hindbrain ISH
Long-pec telencephalon ISH
ptch2 WT standard conditions Prim-15 central nervous system ventral region ISH
gli1ts269/+; gli2aty119/+ standard conditions Prim-15 central nervous system ventral region ISH
gli1ts269/ts269 standard conditions Prim-15 central nervous system ventral region ISH
gli2aty119/ty119 standard conditions Prim-15 central nervous system ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gli1ts269/+; gli2aty119/+ standard conditions Prim-25 somite U-shaped, abnormal
Prim-25 whole organism curved ventral, abnormal
gli1ts269/ts269 standard conditions Prim-25 somite U-shaped, abnormal
Prim-25 whole organism curved ventral, abnormal
gli2aty119/ty119 standard conditions Prim-25 somite U-shaped, abnormal
Prim-25 whole organism curved ventral, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development