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ZFIN ID: ZDB-FIG-050209-6
Karlstrom et al., 2003 - Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development. Development (Cambridge, England)   130(8):1549-1564 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 5 gli1 knockdown phenocopies dtr/gli1. (A) Expression of nk2.2 is unaffected by injection of a control morpholino (MO). (B) dtr/gli1 mutations eliminate nk2.2 expression in some regions of the brain (arrow and arrowhead). (C) Injection of a gli1MO into wild-type embryos leads to a loss of nk2.2 expression identical to that seen in dtr/gli1 mutant embryos (compare arrows, see also Table 1). (D) Expression of fkd4 in the medial and lateral floor plate is unaffected by control MO injections. (E) fkd4 expression is extremely reduced in dtr/gli1 mutants (compare bracket and arrow to those in D). (F) fkd4 expression is similarly reduced in lateral floor plate cells after gli1 MO injection (compare bracket and arrow to those in D). (d′, e′, and f′) show cross sections through the trunk at the level of the yolk plug. nc; notochord

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa WT control Prim-5 lateral floor plate ISH
Prim-5 medial floor plate ISH
WT + MO1-gli1 standard conditions Prim-5 lateral floor plate ISH
Prim-5 medial floor plate ISH
gli1ts269/ts269 control Prim-5 lateral floor plate ISH
Prim-5 medial floor plate ISH
nkx2.2a WT control 20-25 somites brain ISH
20-25 somites forebrain ISH
20-25 somites spinal cord neural tube ISH
WT + MO1-gli1 standard conditions 20-25 somites brain ISH
20-25 somites forebrain ISH
20-25 somites Not Detected spinal cord neural tube ISH
gli1ts269/ts269 control 20-25 somites brain ISH
20-25 somites forebrain ISH
20-25 somites Not Detected spinal cord neural tube ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development