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Fig. 6

Gene expression analysis and visualization of potential marker genes in WT pigment cell clusters. (A) Proportion of cells in WT skin expressing a specific set of genes within each pigment cell cluster. “Neither” denotes the proportion of cells in the cluster that do not meet the minimum threshold for the expression of selected genes. (B) Uniform manifold approximation and projection (UMAP) visualization of pigment cell clusters from WT fish. The data are represented by dots, each corresponding to a single cell, with cells expressing specific genes or coexpressing several genes color coded. (C) Fold change values of differential expression between the WT and the mc2rKO mutant for marker genes identified in each pigment cell type. A negative fold change value indicates overexpression in the WT, whereas positive values denote overexpression in the mc2rKO mutant. All the genes displayed a p value of less than 0.05.

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