Fig. 8
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Fig. 8 Zebrafish embryos that were co-injected with 4 ng splice-blocking dachb-morpholino (dachb-MO-S) + 4 ng p53-MO showed an altered expression of a number of other genes at 30 h post-fertilization. Embryos were either uninjected or else they were injected with 4 ng p53-MO or with 4 ng dachb-MO-S + 4 ng p53-MO and then the reverse transcription polymerase chain reaction was carried out to determine any changes in the expression of various (a) pancreas hormone-related genes; (b,c) regulators of pancreas development; and (c) cell cycle genes.The data are presented as the mean ± standard error of four experiments conducted per treatment. The asterisks indicate p53-MO + dachb-MO data that are significantly different from the p53-MO alone injected controls at P < 0.05* using the Mann–Whitney U-test.