Comparative analysis of dopamine receptor gene expression between wild-type and neu1-knockout zebrafish. A, B Relative mRNA expression levels of (A) nicotinic acetylcholine receptor subunit genes and (B) dopamine receptors in the brains of WT and neu1-KO zebrafish. n = 6. C Effects of Nco exposure on the expression of the dopamine receptor gene in zebrafish brains. WT and neu1-KO zebrafish were exposed to Nco (2.5 mg/L) for 15 min before brain collection. mRNA expression levels were normalized to those of actb and are presented relative to those in WT vehicle controls. n.s., not significant. n = 6. D Double immunofluorescence staining for c-Fos (red) and tyrosine hydroxylase (TH; green) in WT zebrafish following Nco exposure (2.5 mg/L) for 15 min before brain collection. Nuclei were counterstained with DAPI. Arrowheads indicate areas of signal overlap (co-localization) between c-Fos and TH in WT zebrafish following Nco treatment. HC, hypothalamic periventricular complex. Scale bars, 100 µm
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