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Tsuji et al., 2026 - Neu1 deficiency is associated with reduced nicotine responsiveness and dopamine D3 receptor downregulation in zebrafish

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Fig. 5

Comparative analysis of dopamine receptor gene expression between wild-type and neu1-knockout zebrafish. A, B Relative mRNA expression levels of (A) nicotinic acetylcholine receptor subunit genes and (B) dopamine receptors in the brains of WT and neu1-KO zebrafish. n = 6. C Effects of Nco exposure on the expression of the dopamine receptor gene in zebrafish brains. WT and neu1-KO zebrafish were exposed to Nco (2.5 mg/L) for 15 min before brain collection. mRNA expression levels were normalized to those of actb and are presented relative to those in WT vehicle controls. n.s., not significant. n = 6. D Double immunofluorescence staining for c-Fos (red) and tyrosine hydroxylase (TH; green) in WT zebrafish following Nco exposure (2.5 mg/L) for 15 min before brain collection. Nuclei were counterstained with DAPI. Arrowheads indicate areas of signal overlap (co-localization) between c-Fos and TH in WT zebrafish following Nco treatment. HC, hypothalamic periventricular complex. Scale bars, 100 µm

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Fish Physiol. Biochem.