FIGURE

Fig. 3

ID

ZDB-FIG-260710-3

Publication

Tsuji et al., 2026 - Neu1 deficiency is associated with reduced nicotine responsiveness and dopamine D3 receptor downregulation in zebrafish

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Fig. 3

Nicotine-induced neuronal activation in zebrafish brain. A Schematic of the sagittal section of the zebrafish brain. B–E Immunoreactivity of c-Fos in WT and neu1-KO zebrafish brains following Nco exposure, indicating neuronal activation patterns in multiple brain regions. WT and neu1-KO zebrafish were exposed to Nco (2.5 mg/L) for 15 min before brain collection. F–H Quantification of c-Fos fluorescence intensity in the hypothalamus, telencephalon, and diencephalon. n = 3. I Whole-brain c-fos mRNA expression following Nco exposure. n = 6. Fluorescence intensities and c-fos mRNA expression levels are shown relative to those of WT zebrafish without Nco exposure. Scale bars, 100 µm. Different letters indicate statistically significant differences between the groups

Expression Data

Gene:	fosab
Fish:	RW neu1^{zf3377/zf3377} (RW)
Anatomical Terms:	brain diencephalon hypothalamus telencephalon
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	neu1^{zf3377/zf3377} (RW)
Observed In:	brain diencephalon hypothalamus telencephalon
Stage:	Adult

Phenotype Detail

Acknowledgments

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