Fig. 4
- ID
- ZDB-FIG-260507-9
- Publication
- Letelier et al., 2023 - Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network
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Altered expression of Bipolar and Müller glia cell markers in 3dpf vsx mutant fish. (a-h). Confocal sections from in toto in situ hybridization experiments using specific fluorescent probes to label different cell types in wildtype and vsxKO retinas at 72hpf. No clear differences in the expression of the photoreceptor marker prdm1a were observed in ONL of wildtype (a) and mutant samples (e). Bipolar cell marker prkcbb expression (b, f) is considerably reduced in the INL of vsxKO mutant retinas (f, white arrowheads) compared to wildtype (b). Similar expression of the amacrine cell marker ptf1a is observed in the INL from wildtype (c) and vsxKO (g) retinas. Increased expression of the Müller glia cell marker gfap (d, h) is observed in the INL of vsxKO samples (h, white arrowheads) compared to wildtype (d) retinas. ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer. Scale bar in (a-h): 50 µm. |
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| Fish: | |
| Anatomical Terms: | |
| Stage: | Protruding-mouth |
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| Observed In: | |
| Stage: | Protruding-mouth |