FIGURE

Fig. 3

ID

ZDB-FIG-251231-3

Publication

Shi et al., 2025 - Minoxidil ameliorates myelodysplastic syndrome by targeting Wnt4 while sparing normal hematopoiesis

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Fig. 3

Transcriptomic and functional validation of wnt4 as a minoxidil-regulated gene that promotes HSPCs accumulation in zebrafish. A Correlation analysis of 3 control groups and 3 minoxidil-treated groups. B Different expression genes were identified after minoxidil treatment. (q < 0.05, baseMean > 50, log2 fold change > 1). C GO/KEGG analysis of downregulated genes. D Heatmap showing the 8 genes in growth pathway. E WISH of c-myb expression in control, minoxidil-treated, wnt4-overexpressing, and co-treated embryos at 3 dpf. F Quantification of c-myb-positive cells in CHT (Student’s t-test; mean ± SD; ns, not significant; **P < 0.01; ****P < 0.0001). G WISH of c-myb expression in control (left panel) and wnt4-overexpression (right panel) embryos at 60 hpf. H Quantification of c-myb-positive cells in CHT (Student’s t-test; mean ± SD; ****P < 0.0001). I WISH of runx1 expression in control and wnt4-overexpressing embryos at 60 hpf (top) and 3 dpf (bottom); left panels show control, right panels show wnt4 overexpression. J Quantification of runx1-positive cells in CHT (Student’s t-test; mean ± SD; **P < 0.01; ****P < 0.0001; purple dots indicate 60 hpf, orange dots indicate 3 dpf)

Expression Data

Genes:	col4a1 col4a5 ccn1 dcn dvl2 krt17 myb npr2 wnt4
Fish:	la2Tg/la2Tg AB
Condition:	chemical treatment by environment: minoxidil
Anatomical Term:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la2Tg/la2Tg AB
Condition:	chemical treatment by environment: minoxidil
Observed In:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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