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Loss of wnt4 reduces HSPCs numbers and alleviates myeloid hyperplasia in zebrafish. A Sudan Black B (SB) staining of control and wnt4 crispants (wnt4 KO) in both WT and c-mybhyper larvae at 4 dpf. B Quantification of SB-positive neutrophils in the caudal hematopoietic tissue (CHT) (Student’s t-test; mean ± SD; ****P < 0.0001). C WISH of control and wnt4 crispants (wnt4 KO) in both WT and c-mybhyper embryos at 60 hpf. D Quantification of c-myb-positive cells in the CHT at 60 hpf (Student’s t-test; mean ± SD; *P < 0.05; ****P < 0.0001). E WISH of control and wnt4 crispants (wnt4 KO) in both WT and c-mybhyper embryos at 3 dpf. F Quantification of c-myb-positive cells in the CHT at 3 dpf (Student’s t-test; mean ± SD; *P < 0.05; ****P < 0.0001)
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