Fig. 4

Minoxidil and wnt4 loss suppress hematopoiesis and partially reverse MDS-like myeloid expansion. A SB staining of 4 dpf c-myb^hyper embryos treated with increasing concentrations of minoxidil (0, 0.04, 0.4, and 4 mM). B Quantification of SB-positive neutrophils in the CHT region under the indicated conditions (mean ± SD; Student’s t-test; ns, not significant; ***P < 0.001; ****P < 0.0001). C, D WISH of rag1 expression at 4 dpf in WT and c-myb^hyper larvae treated with two concentrations of minoxidil (0.4 mM and 4 mM). Red arrowheads indicate rag1⁺ lymphocytes. Larvae were categorized into high, medium, or low expression groups based on staining intensity. (Chi-square test; replicates = 3, n > 3; ns, not significant; *P < 0.05; ****P < 0.0001). E–F WISH of βe1-globin expression at 4 dpf in WT and c-myb^hyper larvae treated with two concentrations of minoxidil (0.4 mM and 4 mM). Red arrowheads indicate βe1⁺ erythrocytes. Embryos were classified into high, medium, or low expression groups based on staining intensity. (Chi-square test, replicates = 3, n > 3; ns, not significant; ****P < 0.0001)