Fig. 2

Minoxidil inhibits cd41:eGFP^low HSPCs proliferation in zebrafish embryos without triggering apoptosis. A Double staining of bromo deoxyuridine (BrdU) and cd41:eGFP cells in CHT region at 3 dpf. White arrowheads indicate cd41:eGFP^low/BrdU double positive cells, yellow arrowheads indicate cd41:eGFP^high/BrdU double positive cells. Scale bars: 25 μm. B Statistics result of the percentage of cd41:eGFP^low/BrdU double positive cells in total cd41:eGFP^low cells at 3 dpf (Student’s t-test, n = 14 and n = 8, respectively; mean ± SD; *P < 0.05). C Statistics result of the percentage of cd41:eGFP^high/BrdU double positive cells in total cd41:eGFP^high cells at 3 dpf (Student’s t-test, n = 14 and n = 8, respectively; mean ± SD; ns, not significant). D Double staining of TUNEL and cd41:eGFP cells in CHT region at 3 dpf. White arrowheads indicate cd41:eGFP^low/TUNEL double-positive cells. Scale bars: 25 μm. E Quantification of the percentage of cd41:eGFP^low/TUNEL double positive cells in total cd41:eGFP^low cells at 3 dpf (Student’s t-test, n = 13; mean ± SD; ns, not significant). F Quantification of the percentage of cd41:eGFP^high/TUNEL double positive cells in total cd41:eGFP^high cells at 3 dpf (Student’s t-test, n = 13; mean ± SD; ns, not significant)