FIGURE

Fig. 5

ID

ZDB-FIG-251114-5

Publication

Zhao et al., 2025 - β-Glucan pretreatment mitigates Edwardsiella piscicida-induced enteritis through restoring gut microbiota-associated tyrosine metabolism in zebrafish

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Fig. 5

Inhibition of Pah abrogates the protective effects of BG pretreatment. (A) Schematic model of Pah inhibitor administration before BG-pretreatment and bacterial challenge in zebrafish. (B) Transcriptional expression of tight junction protein-encode genes in posterior intestines of PBS- and BG-pretreated zebrafish during E. piscicida infection, with/without Pah inhibitor administration. (C) Histological analysis of posterior intestines in PBS- and BG-pretreated zebrafish during E. piscicida infection, with/without Pah inhibitor administration. Scale bar, 50 μm. (D) Statistical analysis of the histological score as indicated in panel C. (E and F) Transcription of il1b (E) and il6 (F) in posterior intestines of PBS- and BG-pretreated zebrafish during E. piscicida infection, with/without Pah inhibitor administration. Data are replicated with three independent experiments and shown as mean ± SD by a two-way ANOVA with multiple comparisons. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant.

Expression Data

Genes:	cldn1 cldn2 cldna il1b il6 tjp1a
Fish:	AB
Condition:	bacterial treatment by injection: Edwardsiella piscicida, chemical treatment by injection: (1->3,1->6)-beta-D-glucan
Anatomical Term:	posterior intestine
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	bacterial treatment by injection: Edwardsiella piscicida, chemical treatment by injection: (1->3,1->6)-beta-D-glucan
Observed In:	intestinal epithelium intestinal villus intestine lumen posterior intestine
Stage:	Adult

Phenotype Detail

Acknowledgments

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