FIGURE

Figure 3

ID

ZDB-FIG-251023-144

Publication

Ameen et al., 2025 - Mutation of the GDP-Fucose Biosynthesis Gene gmds Increases Hair Cell Number and Neuromast Regenerative Capacity in Zebrafish

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Figure 3

No change in neuromast genetic markers or neural innervation in gmds^−/− mutants. Expression level of the gmds gene at 3 dpf shows downregulation in homozygous mutants when compared to control siblings (A,B). No changes are observed in the expression of slc17a8 (C,D), the neuromast-specific fucosyltransferase fut9b (E,F) or the support cell specific sox2 (G,H) between gmds^−/− and control siblings at 3 dpf. In situ hybridization experiment was repeated three times for each genetic marker (WT n = 15, gmds^−/− n = 15), with pictures at 40X. Neuromasts are innervated, with both gmds^−/− and control siblings staining positive for Hnk-1 (I,J, pictures taken at 200X) and acetylated tubulin (K,L, pictures taken at 630X) using immunohistochemistry from two independent experiments (WT n = 6, gmds^−/− n = 6). Arrows point to nerve innervation in K and L.

Expression Data

Genes:	fut9b.1 gmds slc17a8 sox2
Antibodies:	Ab1-tuba zn-12
Fish:	AB gmds^nfl2/nfl2 (AB)
Anatomical Terms:	neuromast neuromast nerve neuromast hair cell neuromast support cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gmds^nfl2/nfl2 (AB)
Observed In:	neuromast innervation neuromast hair cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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