Figure 5

Effect of socs3b ablation on the innate immune cell response to injury. Wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) embryos on either the Tg(mpx::GFP) or Tg(mpeg1.1::GFP) transgenic background were subjected to a tail wounding assay at 5 dpf and imaged under fluorescence from 0-24 hours post wounding (hpw) (A). Representative images of mpx⁺ neutrophil (B) and mpeg1.1⁺ macrophage (E) cell migration to the injury site (dotted line) at the indicated times, with scale bars representing 100 mm. Quantitation of all migrating mpx⁺(C) and normalized to total mpx⁺ cells (D) as well as all mpeg1.1⁺ cells (F). Gene expression analysis of the indicated genes in WT and KO embryos subjected to wounding presented as fold-change (log₂) relative to 0 hpw (G). Panels (C, D, F and G) show means and SEM, with statistical significance indicated using unpaired t tests (C, D and F) or one-way ANOVA (G) (*** p < 0.001, ** p < 0.01, * p < 0.05, n = 8-12).