FIGURE

Figure 4

ID

ZDB-FIG-230330-23

Publication

Sobah et al., 2023 - Socs3b regulates the development and function of innate immune cells in zebrafish

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Figure 4

Effect of socs3b ablation on neutrophil maturation and functionality. Wildtype (WT, socs3b^wt/wt) and knockout (KO, socs3b^mdu24/mdu24) embryos on the Tg(mpx::GFP) transgenic background at 5 dpf imaged by light (A, B) and fluorescence (C, D) microscopy or subjected to Sudan black staining (E, F), showing representative images in each case. Embryos were also subjected to microinjection with LPS at 5 dpf and imaged by fluorescence microscopy 8 hours later (G–J), showing representative images. Scale bars represent 100 μm. Quantitation of Sudan black⁺(K) or mpx⁺ cells (L) with mean and SEM shown, with significance tested using one-way ANOVA (***p < 0.001, *p < 0.05, n = 8-12).

Expression Data

Gene:	EGFP
Fish:	i114Tg/i114Tg socs3b^mdu24/mdu24; i114Tg/i114Tg
Condition:	chemical treatment by environment: lipopolysaccharide
Anatomical Term:	neutrophil
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg/i114Tg socs3b^mdu24/mdu24; i114Tg/i114Tg
Condition:	chemical treatment by environment: lipopolysaccharide
Observed In:	neutrophil
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol