Fig. 3

The expression of inflammatory mediators and regulators in response to LPS is largely normal in Tollip‐deficient larvae. (A) 24‐h LPS treatment induces the mortality in mutant and wild‐type larvae (3–4 dpf) in a dose‐dependent manner (an average of 4 experiments, 10 larvae per condition). (B–I) 6‐h exposure to LPS (75 µg·mL⁻¹) induces expression of genes encoding NF‐κB components (nfkb2, nfkbiaa), interferon (ifnphi1), proinflammatory cytokines (tnfα, il1b, il6), anti‐inflammatory cytokine (il10), and matrix metalloproteinase mmp9 in wild‐type and tollip^−/− 3 dpf larvae. Each dot represents data from one independent experiment (encompassing a pool of 10 larvae/condition), whereas bars represent the means ± SEM from n = 4 experiments. (A) Mann–Whitney U test, *P = 0.571; (B–I) ANOVA after log‐transforming the data, followed by Dunnett’s post hoc test, ns.—nonsignificant, *P < 0.01; **P < 0.005; ***P < 0.001.