ZFIN ID: ZDB-FIG-220527-5
Gao et al., 2022 - Accumulation of Lipid Droplets in a Novel Bietti Crystalline Dystrophy Zebrafish Model With Impaired PPARα Pathway. Investigative ophthalmology & visual science   63:32 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Downregulation of the PPARα pathway was involved in cyp4v7/cyp4v8 DKO zebrafish RPE cells. (A) Volcano plot of differentially expressed genes in cyp4v7−/−cyp4v8−/− zebrafish compared with WT. (B) KEGG circle enrichment analysis of the differentially expressed genes. The first lap indicates the top 20 KEGG terms. The second lap represents the gene numbers in the genome backdrop. The third circle represents the ratio of the upregulated genes (dark purple) and downregulated genes (light purple). The fourth circle represents the enrichment element of each KEGG term. (C) KEGG enriched metabolism pathways (P < 0.05) are shown. (D) Expression patterns of genes in PPARα signaling pathway in WT and cyp4v7−/−cyp4v8−/− zebrafish RPE cells are shown in the heatmap. (E) Validation of the expression of genes in PPARα pathway by qPCR in cyp4v7 and cyp4v8 DKO zebrafish RPE cells at seven months after fertilization. (F) Detection of the protein level of PPARα in WT and cyp4v7−/−cyp4v8−/− zebrafish RPE cells by Western blot. The results are shown as mean ± SD. ***P < 0.001; **P < 0.01; *P < 0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acsl1a WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
apoa1a WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
cyp27a1.2 WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
fabp4a WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
fabp7a WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
fads2 WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
hacl1 WT standard conditions Adult whole organism RTPCR
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-ppara WB WT standard conditions Adult whole organism
WB cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism
Phenotype Details
Fish Conditions Stage Phenotype
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism acsl1a expression decreased amount, abnormal
Adult whole organism apoa1a expression decreased amount, abnormal
Adult whole organism cyp27a1.2 expression decreased amount, abnormal
Adult whole organism fabp4a expression decreased amount, abnormal
Adult whole organism fabp7a expression decreased amount, abnormal
Adult whole organism fads2 expression decreased amount, abnormal
Adult whole organism hacl1 expression decreased amount, abnormal
Adult whole organism Ab6-ppara labeling decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.