ZFIN ID: ZDB-FIG-220527-4
Gao et al., 2022 - Accumulation of Lipid Droplets in a Novel Bietti Crystalline Dystrophy Zebrafish Model With Impaired PPARα Pathway. Investigative ophthalmology & visual science   63:32 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4

Lipid accumulation in RPE cells of WT and cyp4v7−/−cyp4v8−/− zebrafish. (A) Nile red staining of lipid droplets in retinal frozen sections of WT and cyp4v7−/−cyp4v8−/− zebrafish at 3, 7, 10, and 12 mpf. White arrows, lipid droplets. Scale bar: 20 µm. (B) Nile red staining of RPE flat mounts from WT and cyp4v7−/−cyp4v8−/− zebrafish at 3, 7, and 10 months after fertilization. White arrows, lipid droplets (bright red). Scale bar: 20 µm. Bar graphs show LD size distribution in cyp4v7−/−cyp4v8−/− zebrafish RPE flat mounts at 3, 7, and 10 months after fertilization. Diameter of LDs in cyp4v7−/−cyp4v8−/− zebrafish RPE cell was measured and represented by white (>3 μm), black (2-3 μm), red (1-2 μm), and blue (0-1 μm) (n = 88-103 RPE cells examined over three biologically independent experiments). (C) TEM images of retinal ultrathin sections from WT and cyp4v7−/−cyp4v8−/− zebrafish at seven months after fertilization. Lipid droplets are labeled with red text “LD”. Vacuoles are labeled with red text “V.”

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult retinal pigmented epithelium lipid droplet present, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.