ZFIN ID: ZDB-FIG-220527-2
Gao et al., 2022 - Accumulation of Lipid Droplets in a Novel Bietti Crystalline Dystrophy Zebrafish Model With Impaired PPARα Pathway. Investigative ophthalmology & visual science   63:32 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 2

 
Progressive degeneration of photoreceptor cells in cyp4v7/cyp4v8 DKO zebrafish. (A) Histological analysis revealed the progressive degeneration of the outer retinal layer in cyp4v7−/−cyp4v8−/− zebrafish. OS, outer segment; IS, inner segment; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bars: 20 µm. (B) The quantitative results of the thickness of OS layer (represented by the length from the lower edge of IS to the upper edge of RPE) in A. Three parallel samples were tested for each group. The results are shown as mean ± SD. ***P < 0.001; **P < 0.01. (C) Western blotting analysis of Gnat1 and Gnat2 protein levels in WT and mutant zebrafish retinas at 10 and 12 months after fertilization. Tubulin was used to normalize protein loading. (D) Quantitative analysis of the Western blot data. At least three independent experiments were performed and quantified. The results are shown as mean ± SD. ***P < 0.001; **P < 0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gnat1 Ab1-gnat1 WT standard conditions Adult whole organism WB
Ab1-gnat1 cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-gnat2 WB WT standard conditions Adult whole organism
WB cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult whole organism
Phenotype Details
Fish Conditions Stage Phenotype
cyp4v2ahzu15/hzu15; cyp4v2bhzu14/hzu14 standard conditions Adult photoreceptor outer segment layer decreased thickness, abnormal
Adult retinal photoreceptor layer degenerate, abnormal
Adult whole organism gnat1 expression decreased amount, abnormal
Adult whole organism ab2-gnat2 labeling decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.