FIGURE

Figure 1

ID

ZDB-FIG-210902-88

Publication

Sun et al., 2021 - Identification of Potentially Related Genes and Mechanisms Involved in Skeletal Muscle Atrophy Induced by Excessive Exercise in Zebrafish

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Figure 1

Excessive exercise causes skeletal muscle atrophy in zebrafish. (A) Representative image of excessive exercise and control zebrafish. (B) Changes in the body weight of zebrafish with or without excessive exercise over a 4-week period. (C) Representative photomicrographs of muscle sections stained with H&E. Scale bars = 20 μm. (D) Average fiber size (based on H&E staining). (E) The expression of muscle-atrophy-related genes (trim63a, trim63b, fbxo25, fbxo32, capn1, and casp3a), cyclin (ccnd1, ccne1, and ccng2), mitochondrial function markers (cyc1, nd4, and nd5), and ppargc1a in muscle tissue detected using real-time PCR. (F–I) Western blot analysis of Murf and Fbox32 proteins (representative blot). All uncut western blot images are provided in Supplementary Materials. Data represent mean ± SD (n = 6). (J,K) The critical swimming speed (U_crit) and oxygen consumption (MO₂) were measured using the Loligo System. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Expression Data

Genes:	capn1 casp3a ccnd1 ccne1 ccng2 cyc1 fbxo25 fbxo32 mt-nd4 mt-nd5 ppargc1a trim63a trim63b
Fish:	WT
Condition:	through flow
Anatomical Term:	skeletal muscle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	through flow
Observed In:	aerobic respiration skeletal muscle skeletal muscle cell swimming whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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