FIGURE

Fig. 5

ID

ZDB-FIG-200227-5

Publication

Chen et al., 2020 - The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond-Blackfan anemia

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Fig. 5

Upregulation of p53 activity partially contributed to the erythroid phenotype in <italic>rpl18</italic> mutants.

a Transcript quantification of p53 and p53 related genes were verified by qRT-PCR. All the p53 related genes were highly expressed in rpl18 mutants at 30 hpf (N = 3), *P < 0.05. **P < 0.01. b WISH showed that p53, p21, and mdm2 expression was elevated in rpl18 mutants at 30 hpf (N = 3). c In situ cell death staining result showed that the signal of apoptosis can be observed thought the body at 30 and 48 hpf (N = 4). d An increase of hemoglobin level appeared in mutants at 3 dpf after p53 knockdown (N = 3). Arrowhead points to the recovery of erythrocytes. All scale bars represent 250 μm.

Expression Data

Genes:	baxa bbc3 casp8 ccng1 cdkn1a fas mdm2 tp53
Fish:	rpl18^{zf3219/zf3219} (TU)
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpl18^{zf3219/zf3219} (TU) rpl18^{zf3219/zf3219} + MO4-tp53 (TU)
Knockdown Reagent:	MO4-tp53
Observed In:	whole organism whole organism apoptotic process whole organism hemoglobin
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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