FIGURE

Fig. 2

ID

ZDB-FIG-190201-6

Publication

Naylor et al., 2018 - A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion

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Fig. 2

CS extrusion is mediated by apical constriction.

(A) Panels show lateral views of the markers indicated in the region of the trunk where the CS will be derived (at 22 hpf) and is extruded (at 50 hpf). (B) Panels show lateral views of the trunk from live Tg(cdh17:egfp) embryos (left) and embryos stained for cdh17 (red)/stc1 (purple) transcripts (right) in control and Blebbistatin treated embryos. Black arrow indicates the position of stc1⁺ cells in the Blebbistatin treated embryo. (C) Panels show brightfield/fluorescence in Tg(cdh17:egfp) embryos before and after laser ablation (site of ablation indicated with asterisk). Panels on the right show embryos 18 hr post-ablation and arrowheads indicate the extruded CS gland.

Expression Data

Genes:	atp1a1a.4 cdh17 EGFP krt18a.1 slc12a1 stc1
Fish:	TU nz1Tg
Condition:	chemical treatment by environment: blebbistatin
Anatomical Terms:	corpuscles of Stannius pronephric distal early tubule
Stage Range:	26+ somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU
Condition:	chemical treatment by environment: blebbistatin
Observed In:	corpuscles of Stannius corpuscles of Stannius embryonic organ development pronephric distal early tubule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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