|ZFIN ID: ZDB-IMAGE-180621-37|
A phosphorylation-resistant Yap1 mRNA (caYap1) rescued the pLLP and NM phenotype of Lpar2b morphants. (A–C″) Confocal images of the pLLP stained with DAPI (blue) and BrdU (red) at 32 hpf in Et(gata2:EGFP)mp189b embryos injected with control MO (A–A″), lpar2b MO (B–B″) or lpar2b MO and caYap1 mRNA together (C–C″). The pLLP is outlined by dots. (D–F) Epifluorescence image s of Et(gata2:EGFP)mp189b embryos injected with control MO (D), lpar2b MO (E), or lpar2b MO and caYap1 mRNA together (F) at 48 hpf. (G–I) Quantification of the total number of cells (G) and the number of BrdU+ cells (H) in the pLLP at 32 hpf, and the number of NMs at 48 hpf (I) in 3 groups of embryos as indicated. *p < 0.05; **p < 0.01.
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