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ZFIN ID: ZDB-FIG-180620-6
Gou et al., 2018 - sox2 and sox3 play unique roles in development of hair cells and neurons in the zebrafish inner ear. Developmental Biology   435(1):73-83 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 6

Axial patterning in the otic vesicle following early high-level misexpression ofsox2orsox3. Dorsolateral views (anterior to the left) showing expression of various regional markers at 24 hpf in the otic vesicle of control (A–J), hs:sox2/hs:sox2 (A’–J’) and hs:sox3/hs:sox3 (A’’–J’’) embryos following heat-shock at 12.5 hpf. hs:sox2/hs:sox2 embryos were heat shocked at 39 °C for 60 min, hs:sox3/hs:sox3 embryos were heat shocked at 38 °C for 30 min. Otic vesicle borders are outlined in all images.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 Not Detected otic vesicle ISH
fgf3 AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
fgf8a AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
gsc AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 Not Detected otic vesicle ISH
hmx3a AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
otx1 AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 Not Detected otic vesicle ISH
pax2a AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
pax5 AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
pou3f3b AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 Not Detected otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 Not Detected otic vesicle ISH
tbx1 AB control Prim-5 otic vesicle ISH
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle ISH
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
x21Tg/x21Tg(AB) heat shock Prim-5 otic vesicle pou3f3b expression absent, abnormal
Prim-5 otic vesicle gsc expression decreased amount, abnormal
Prim-5 otic vesicle dlx3b expression decreased amount, abnormal
Prim-5 otic vesicle tbx1 expression decreased amount, abnormal
Prim-5 otic vesicle otx1 expression decreased amount, abnormal
Prim-5 otic vesicle tbx1 expression decreased distribution, abnormal
Prim-5 otic vesicle otx1 expression decreased distribution, abnormal
Prim-5 otic vesicle dlx3b expression decreased distribution, abnormal
Prim-5 otic vesicle gsc expression decreased distribution, abnormal
Prim-5 otic vesicle pax2a expression increased amount, abnormal
Prim-5 otic vesicle fgf3 expression increased amount, abnormal
Prim-5 otic vesicle pax5 expression increased amount, abnormal
Prim-5 otic vesicle hmx3a expression increased amount, abnormal
Prim-5 otic vesicle fgf8a expression increased amount, abnormal
Prim-5 otic vesicle hmx3a expression increased distribution, abnormal
Prim-5 otic vesicle fgf3 expression increased distribution, abnormal
Prim-5 otic vesicle pax2a expression increased distribution, abnormal
Prim-5 otic vesicle fgf8a expression spatial pattern, abnormal
Prim-5 otic vesicle pax5 expression spatial pattern, abnormal
Prim-5 otic vesicle fgf3 expression spatial pattern, abnormal
Prim-5 otic vesicle pax2a expression spatial pattern, abnormal
Prim-5 otic vesicle hmx3a expression spatial pattern, abnormal
x32Tg/x32Tg(AB) heat shock Prim-5 otic vesicle dlx3b expression absent, abnormal
Prim-5 otic vesicle otx1 expression absent, abnormal
Prim-5 otic vesicle pou3f3b expression absent, abnormal
Prim-5 otic vesicle gsc expression absent, abnormal
Prim-5 otic vesicle tbx1 expression decreased amount, abnormal
Prim-5 otic vesicle tbx1 expression decreased distribution, abnormal
Prim-5 otic vesicle pax5 expression increased amount, abnormal
Prim-5 otic vesicle hmx3a expression increased amount, abnormal
Prim-5 otic vesicle fgf8a expression increased amount, abnormal
Prim-5 otic vesicle fgf3 expression increased amount, abnormal
Prim-5 otic vesicle pax2a expression increased amount, abnormal
Prim-5 otic vesicle hmx3a expression increased distribution, abnormal
Prim-5 otic vesicle pax2a expression increased distribution, abnormal
Prim-5 otic vesicle fgf3 expression increased distribution, abnormal
Prim-5 otic vesicle fgf8a expression spatial pattern, abnormal
Prim-5 otic vesicle hmx3a expression spatial pattern, abnormal
Prim-5 otic vesicle pax2a expression spatial pattern, abnormal
Prim-5 otic vesicle fgf3 expression spatial pattern, abnormal
Prim-5 otic vesicle pax5 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 435(1), Gou, Y., Vemaraju, S., Sweet, E.M., Kwon, H.J., Riley, B.B., sox2 and sox3 play unique roles in development of hair cells and neurons in the zebrafish inner ear, 73-83, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.