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ZFIN ID: ZDB-FIG-180522-28
Duong et al., 2017 - Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms. Developmental Biology   434(1):7-14 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 3

AVC markers are expanded innr2f1amutant hearts. (A-L) ISH for bmp4 (A,B), tbx2b (C,D), has2 (E,F), klf2a (G,H), notch1b (I,J), and spp1 (K,L) in WT and nr2f1a mutant embryos. Images are frontal views with dorsal up. The relative position of the ventricle and atrium is the same in all images. Arrowheads indicate the length of expression on the superior side (s) of the AVC, which is adjacent to the inner curvature of the ventricle. Inferior side (i) of the AVC is adjacent to the outer curvature of the ventricle. (M) Length measurements of marker expression in the AVC. (WT bmp4 n = 7, nr2f1a-/-bmp4 n = 7, WT tbx2b n = 10, nr2f1a-/-tbx2b n = 10, WT has n = 7, nr2f1a-/-has n = 7, WT klf2a n = 6, nr2f1a-/-klf2a n = 6, WT notch1b n = 5, nr2f1a-/-notch1b n = 5, WT spp1 n = 9, nr2f1a-/-klf2a n = 9.).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
has2 WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
klf2a WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
notch1b WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
spp1 WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
tbx2b WT standard conditions Long-pec atrioventricular canal ISH
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nr2f1ael512/el512 standard conditions Long-pec atrioventricular canal spp1 expression increased distribution, abnormal
Long-pec atrioventricular canal tbx2b expression increased distribution, abnormal
Long-pec atrioventricular canal has2 expression increased distribution, abnormal
Long-pec atrioventricular canal bmp4 expression increased distribution, abnormal
Long-pec atrioventricular canal notch1b expression increased distribution, abnormal
Long-pec atrioventricular canal klf2a expression increased distribution, abnormal
Long-pec atrioventricular canal spp1 expression spatial pattern, abnormal
Long-pec atrioventricular canal has2 expression spatial pattern, abnormal
Long-pec atrioventricular canal tbx2b expression spatial pattern, abnormal
Long-pec atrioventricular canal klf2a expression spatial pattern, abnormal
Long-pec atrioventricular canal notch1b expression spatial pattern, abnormal
Long-pec atrioventricular canal bmp4 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 434(1), Duong, T.B., Ravisankar, P., Song, Y.C., Gafranek, J.T., Rydeen, A.B., Dohn, T.E., Barske, L.A., Crump, J.G., Waxman, J.S., Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms, 7-14, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.