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ZFIN ID: ZDB-FIG-180522-26
Duong et al., 2017 - Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms. Developmental Biology   434(1):7-14 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 1

Nr2f1a is expressed in ACs and generation of anr2f1amutant allele. (A,B) IHC for MHC (red), AHMC (purple), and Nr2f1a (green) at 24 and 48 hpf. Nr2f1a+ nuclei in cells at the venous pole adjacent to AMHC+ cells (yellow arrowheads), which we propose are putatively atrial progenitors. Expression in nuclei of ACs (white arrows). Blue brackets indicate AMHC+ region devoid of Nr2f1+ nuclei. Images are Z-stacks of confocal sections. V indicates ventricle and A indicates atrium in all figures. Scale bar indicates 10 µm in A,B. ≥ 10 hearts were examined at each stage. (C) Schematic of deletion in nr2f1a exon 1 from the TALENs. (D) Sequence of the 8-bp deletion in the nr2f1ael512 mutant allele. (E) Schematic indicating the predicted truncation caused by the deletion. Activation domain – AF1 (red), DNA-binding domain – DBD (green), Ligand binding domain – LBD (yellow). Black indicates amino acids after the protein goes out of frame. (F,G) WT and nr2f1ael512-/- mutant embryos at 48 hpf. (H) Western blot indicating that Nr2f1a protein is lost in nr2f1a mutants. (I,J) Confocal images of whole mount IHC indicating Nr2f1a protein is expressed in the same anatomical structure as shown previously with ISH (Love and Prince, 2012) and is lost in nr2f1a mutants. Images are the dorsolateral with anterior left. e – eyes, p – pharyngeal (arrow in I), c – cerebellum, hb –hindbrain.>20 wt sibling and nr2f1a mutant embryos were examined.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nr2f1a Ab1-nr2f1a WT standard conditions Prim-5 cardiac muscle cell nucleus IHC
Ab1-nr2f1a Long-pec atrium cardiac muscle cell IHC
Ab1-nr2f1a Long-pec atrium cardiac muscle myoblast IHC
Ab1-nr2f1a Long-pec cardiac muscle cell nucleus IHC
Ab1-nr2f1a Long-pec cerebellum IHC
Ab1-nr2f1a Long-pec hindbrain IHC
Ab1-nr2f1a Long-pec pharyngeal arch IHC
Ab1-nr2f1a nr2f1ael512/el512 standard conditions Long-pec Not Detected cerebellum IHC
Ab1-nr2f1a Long-pec Not Detected hindbrain IHC
Ab1-nr2f1a Long-pec Not Detected pharyngeal arch IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nr2f1ael512/el512 standard conditions Long-pec cerebellum nr2f1a expression absent, abnormal
Long-pec hindbrain nr2f1a expression absent, abnormal
Long-pec pericardium edematous, abnormal
Long-pec pharyngeal arch nr2f1a expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 434(1), Duong, T.B., Ravisankar, P., Song, Y.C., Gafranek, J.T., Rydeen, A.B., Dohn, T.E., Barske, L.A., Crump, J.G., Waxman, J.S., Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms, 7-14, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.