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ZFIN ID: ZDB-FIG-180522-27
Duong et al., 2017 - Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms. Developmental Biology   434(1):7-14 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

Nr2f1amutants have smaller atria. (A,B) IHC for MHC (red) and AMHC (green). Sharp border between MHC and AMHC in WT embryos (arrowhead in A). Diffuse border of AMHC expression (arrowheads in B). (C) Number of ACs and VCs in WT sibling (n = 12) and nr2f1a mutant (n = 14) embryos. (D,E) ISH for amhc at the 22 s stage. Images are dorsal views with anterior up. (F,G) ISH for amhc at 48 hpf. Border of expression (arrowheads). (H) RT-qPCR for amhc and vmhc in WT sibling and nr2f1a mutant embryos. (I,J) ISH for vmhc at 48 hpf. Distinct border of expression (arrowhead in I). Expansion and diffuse border of expression (arrowheads in J). Images in F,G,I,J are frontal views.>20 embryos per condition were examined for A,B,D,E,F,G,I,J. (K) Amount of green+/red- cells (area) at the venous (atrium) and arterial (ventricular) poles in WT and nr2f1a mutant embryos. (L-M″) Confocal images of hearts depicting the addition of differentiating cells (green+/red- cells) at the venous and arterial poles (yellow and white arrowheads, respectively). Asterisks indicates significant difference (P<0.05) in all figures.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT standard conditions 20-25 somites heart rudiment ISH
Long-pec atrium cardiac muscle cell ISH
nr2f1ael512/el512 standard conditions 20-25 somites heart rudiment ISH
Long-pec atrium ISH
myh7 WT standard conditions Long-pec cardiac ventricle ISH
nr2f1ael512/el512 standard conditions Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nr2f1ael512/el512 standard conditions Long-pec atrium myh6 expression decreased amount, abnormal
Long-pec atrium decreased size, abnormal
Long-pec atrium cardiac muscle cell decreased amount, abnormal
Long-pec cardiac ventricle myh7 expression increased distribution, abnormal
Long-pec cardiac ventricle myh7 expression spatial pattern, abnormal
Long-pec cardiac ventricle cardiac muscle cell amount, normal
sd22Tg ; nr2f1ael512/el512 standard conditions Long-pec atrial cardiac muscle cell differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 434(1), Duong, T.B., Ravisankar, P., Song, Y.C., Gafranek, J.T., Rydeen, A.B., Dohn, T.E., Barske, L.A., Crump, J.G., Waxman, J.S., Nr2f1a balances atrial chamber and atrioventricular canal size via BMP signaling-independent and -dependent mechanisms, 7-14, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.